{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:56:19Z","timestamp":1790556979735,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Optical autofluorescence imaging is a noninvasive adjunct for detecting potentially malignant oral disorders, such as actinic cheilitis (AC). This study evaluated its use for early detection and for monitoring tissue response following methylene blue\u2010mediated photodynamic therapy (MB\u2010PDT).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Lip autofluorescence was assessed using a handheld device (405\u2009\u00b1\u200920\u2009nm). Areas with altered fluorescence underwent biopsy for confirmation. Patients with AC received five MB\u2010PDT sessions (660\u2009nm, 100\u2009mW, 120\u2009s\/point) after topical 1% MB. Grayscale intensity was quantified before treatment and at 30\u2009days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 25 screened individuals, 19 had confirmed AC, and 17 completed follow\u2010up. Lesions showed higher fluorescence intensity than healthy tissue. After MB\u2010PDT, mean intensity and variability significantly decreased, indicating tissue response. Smokers showed a smaller reduction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Autofluorescence imaging enables early detection and noninvasive monitoring of AC. Combined with MB\u2010PDT, it represents a practical strategy for managing high\u2010risk patients and supporting early intervention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbio.70346","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:21:02Z","timestamp":1788222062000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Autofluorescence Imaging and Methylene Blue\u2010Mediated Photodynamic Therapy for Early Detection and Management of Subclinical Actinic Cheilitis"],"prefix":"10.1002","volume":"19","author":[{"given":"Juliana Cristina Oliverio","family":"de Ara\u00fajo","sequence":"first","affiliation":[{"name":"Professional Master Radiation Technology in Health Sciences Nuclear and Energy Research Institute (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"},{"name":"Center for Lasers and Applications, Nuclear and Energy Research Institute (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Og Fray","family":"Junior","sequence":"additional","affiliation":[{"name":"Center for Lasers and Applications, Nuclear and Energy Research Institute (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermano Camelo","family":"Paiva","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry School of Dentistry, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4873-1393","authenticated-orcid":false,"given":"Alyne Sim\u00f5es","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Oral Biology School of Dentistry, University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4203-1134","authenticated-orcid":false,"given":"Martha Sim\u00f5es","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Professional Master Radiation Technology in Health Sciences Nuclear and Energy Research Institute (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"},{"name":"Center for Lasers and Applications, Nuclear and Energy Research Institute (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.805425"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-023-01543-z"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13200"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2021.05.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2024.105220"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jormas.2025.102227"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19084593"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-019-02895-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13133354"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1572-1000(05)00097-9"},{"issue":"1","key":"e_1_2_9_12_1","first-page":"14","article-title":"Sample Size Calculation in Medical Studies","volume":"6","author":"Pourhoseingholi M. A.","year":"2013","journal-title":"Gastroenterology and Hepatology From Bed to Bench"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-644020256302"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2019.10.005"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2025.101978"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2019.05.017"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2022.105941"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9061987"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202300414"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2025.106061"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2018.04.008"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/lio2.772"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1264961"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19041107"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-014-1609-1"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e31824f632d"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13613"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-200310000-00005"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/61\/7\/R57"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70346","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.70346","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:21:05Z","timestamp":1790554865000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.70346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/jbio.70346"],"URL":"https:\/\/doi.org\/10.1002\/jbio.70346","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"assertion":[{"value":"2026-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70346"}}