{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:49:20Z","timestamp":1790009360702,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,13]],"date-time":"2004-09-13T00:00:00Z","timestamp":1095033600000},"content-version":"vor","delay-in-days":4153,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Mater. Res."],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It was previously reported that polystyrene substituted with the sulfonate group, PSSO<jats:sub>3<\/jats:sub>, which has anticoagulant heparin\u2010like properties, and then coated with fibronectin supports the growth of human umbilical vein endothelial cells. On the other hand, polystyrene substituted with the amino acid sulfamide group, PSSO<jats:sub>2<\/jats:sub>\u2010Asp, which has a higher anticoagulant activity, and then coated with fibronectin no longer supported the growth of endothelial cells. We report here that, while the affinity of fibronectin to either polymer is of the same order of magnitude, fibronectin is adsorbed onto the PSSO<jats:sub>2<\/jats:sub>\u2010Asp polymer in a different conformation compared to the PSSO<jats:sub>3<\/jats:sub> polymer. This was shown by a higher binding of polyclonal antifibronectin antibodies to fibronectin\u2010coated PSSO<jats:sub>2<\/jats:sub>\u2010Asp polymer, and by a decreased susceptibility of the coated fibronectin to proteolysis by thermolysin. This study provides evidence that a solid phase substrate with a strong heparin\u2010like function may influence the conformation and biological properties of fibronectin. \u00a9 1993 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/jbm.820270509","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T19:26:22Z","timestamp":1117221982000},"page":"619-626","source":"Crossref","is-referenced-by-count":27,"title":["Conformational changes of fibronectin induced by polystyrene derivatives with a heparin\u2010like function"],"prefix":"10.1002","volume":"27","author":[{"given":"L.","family":"Stanislawski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Serne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Stanislawski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jozefowicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1163\/156856290X00017"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02364219"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02364220"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(85)90085-7"},{"key":"e_1_2_1_6_2","first-page":"847","volume-title":"Progress in Artificial Organs 1983","author":"Migonney V.","year":"1984"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820270412"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1242\/jcs.82.1.263","article-title":"Either exogenous or endogenous fibronectin can promote adherence of human endothelial cells","volume":"82","author":"Clark R. 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