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Using detection by colorimetric techniques, soluble preparations were shown to hydrolyze enzyme substrates that are characteristic for trypsinlike proteases, cathepsin\u2010like proteases, and collagenase. As detected by sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis in gradient gels and gel filtration on Sepharose CL\u20106B, insoluble (pellet) preparations degraded denatured type I collagen in a time\u2010dependent pattern, producing low\u2010molecular\u2010weight fragments. These activities were partially inhibited by phenylmethylsulfonyl fluoride, <jats:italic>N<\/jats:italic>\u2010ethyl maleimide, soybean trypsin inhibitor, parachloromercurybenzoic acid, or ethylenediaminetetraacetic acid, suggesting the presence of a heterogeneous enzymatic mixture. Insoluble preparations incubated with pure pericardial dermatan sulfate proteoglycan detached the glycosaminoglycan chains from their core protein carrier, producing a digestion pattern similar to Cathepsin C. These findings demonstrate the presence of active proteases in glutaraldehydefixed bovine pericardium per se and in explanted pericardial bioprosthetic cardiac valves, an additional factor that might contribute to intrinsic extracelluar matrix degeneration in pericardial bioprosthetic devices. \u00a9 1993 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/jbm.820270615","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T19:26:34Z","timestamp":1117221994000},"page":"821-829","source":"Crossref","is-referenced-by-count":24,"title":["Detection of remnant proteolytic activities in unimplanted glutaraldehyde\u2010treated bovine pericardium and explanted cardiac bioprostheses"],"prefix":"10.1002","volume":"27","author":[{"given":"Dan","family":"Simionescu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agneta","family":"Simionescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radu","family":"Deac","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,13]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"105","article-title":"Mechanical characteristics of pericardial tissue and their relevance to bioprosthetic design","volume":"5","author":"Trowbridge E. 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