{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:16:21Z","timestamp":1790057781743,"version":"4.0.1"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,9,13]],"date-time":"2004-09-13T00:00:00Z","timestamp":1095033600000},"content-version":"vor","delay-in-days":3574,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biomed. Mater. Res."],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Early studies had indicated that tissue repair is intially associated with a lower than normal serum pH that later becomes more alkaline. To determine how tissue pH may affect skeletal healing and mineralization, we used a rat skeletal repair model consisting of a long bone segmental defect grafted with acid\u2010demineralized bone matrix (DBM), a biomaterial possessing both osteoinductive and osteoconductive repair properties. In this study, femoral and tibial diaphyses from young adult Sprague Dawley rats were cut into cylinders approximately 0.5 cm in length, demineralized in acid, perforated to accommodate a needle\u2010type combination pH microelectrode, and grafted around a 0.3\u2010cm\u2010long diaphyseal fibula defect. The pH of repair tissues was recorded at various time intervals up to 28 days postgrafting. Healing and mineralization were monitored histologically and by the ash and calcium content of repair tissues. During the early healing phase, tissue pH was lower than normal serum pH, presumably because of an accumulation of acidic metabolites in tissue fluids. Subsequent pH increases to more alkaline values were accompanied by a rapid mineral deposition phase and a later phase characterized by a slow, gradual increase in tissue calcium content. The results of this study support previous observations suggesting that the pH of repair tissue fluids may play a regulatory role in the healing and mineralization of bone. \u00a9 1994 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/jbm.820281209","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T19:30:06Z","timestamp":1117222206000},"page":"1439-1443","source":"Crossref","is-referenced-by-count":79,"title":["Mineralization and pH relationships in healing skeletal defects grafted with demineralized bone matrix"],"prefix":"10.1002","volume":"28","author":[{"given":"D. A.","family":"Chakkalakal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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(Suppl. 1) 733 S\u2010267(1991)."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(71)90127-9"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"903","DOI":"10.2106\/00004623-196749050-00007","article-title":"The effect of reduced barometric pressure on fracture healing in rats","volume":"49","author":"Makley J. T.","year":"1967","journal-title":"J. Bone Jt. Surg."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0371-1951(60)80089-6"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67143-1"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345710500060601"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(72)90475-5"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1383(87)90006-4"},{"key":"e_1_2_1_19_2","first-page":"316","article-title":"Lysozymes in calcifying tissue","volume":"112","author":"Kuettner K. 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