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However, in terms of bone pathology, which is among the most disabling manifestations, a slow and incomplete response is observed, indicating that adjuvant therapies are necessary to consistently restore GCR activity in bone and accelerate regeneration. In this study, calcium alginate microspheres were analyzed as a vehicle for localized GCR delivery to bone. Results demonstrated that the entrapped enzyme retained full activity and exhibited a broader pH\u2010dependent activity profile, compared to that of free\u2010GCR, with improved stability at physiological pH. GCR release profile was established, and it was demonstrated that GCR could be released in a sustained manner. The biological behavior of the system was evaluated by analyzing the uptake of released GCR by GCR\u2010deficient cells from GD patients, using different techniques: GCR activity measurements, radiolabeling, and cellulose acetate electrophoresis. Results demonstrated that GCR was internalized by cells significantly enhancing the residual enzymatic activity. To achieve an activity reconstitution level comparable to that obtained using free\u2010GCR, only half of the dose was required with entrapped\u2010GCR. \u00a9 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005<\/jats:p>","DOI":"10.1002\/jbm.a.30348","type":"journal-article","created":{"date-parts":[[2005,7,18]],"date-time":"2005-07-18T18:46:39Z","timestamp":1121712399000},"page":"545-552","source":"Crossref","is-referenced-by-count":42,"title":["Biological evaluation of calcium alginate microspheres as a vehicle for the localized delivery of a therapeutic enzyme"],"prefix":"10.1002","volume":"74A","author":[{"given":"C. C.","family":"Barrias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lamghari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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