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The surface was highly inert towards coagulation, unfortunately at the expense of increased complement activation. We, therefore, as an alternative approach, used End\u2010Group Activated Pluronic to conjugate factor H, a regulator of complement activation (RCA), to the surface. The bound factor H did not detach from the surface upon incubation with human serum. Furthermore, factor H bound in a physiological conformation could to a significant degree attenuate complement activation at the Pluronic\u2122 surface. Thus, we have created a hybrid surface in which the coagulation\u2010inert properties of the original Pluronic\u2122 are supplemented with a specific complement\u2010inhibitory effect. Medical device technology includes numerous potential applications for crosslinkers that are capable of specifically binding biomolecules to surfaces with retained activity. These applications include coupling of functional biomolecules to biomedical devices such as stents and grafts. The biomolecule may be an RCA, antibody, or other beneficial ligand. \u00a9 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006<\/jats:p>","DOI":"10.1002\/jbm.a.30377","type":"journal-article","created":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T21:16:48Z","timestamp":1130275008000},"page":"25-34","source":"Crossref","is-referenced-by-count":33,"title":["Surface\u2010attached PEO in the form of activated pluronic with immobilized factor H reduces both coagulation and complement activation in a whole\u2010blood 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