{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:12:45Z","timestamp":1697436765808},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,4,6]],"date-time":"2006-04-06T00:00:00Z","timestamp":1144281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human leukocytes (peripheral blood mononuclear cells, PBMC) were overlaid on calcium phosphate bone cement (CBC, Norian SRS\u00ae) and allowed to settle for 1 h under cell culture conditions. Subsequently, the cells were either left unstimulated (i.e. sham stimulation using cell culture medium), or stimulated with toxic shock syndrome toxin\u20101 (TSST\u20101, 10 ng\/mL), staphylococcal enterotoxin B (SEB, 10 ng\/mL), or concanavalin A (ConA, 2 \u03bcg\/mL) for further 24 h using cell culture conditions. Supernatants were then analyzed for cytokine content (interleukin\u20101 receptor antagonist, IL\u20101ra; IL\u20102; IL\u20106; IL\u201010; IL\u201012) by enzyme\u2010linked immunosorbent assay. While the spontaneous generation of cytokines was not influenced, the IL\u20102 release from stimulated PBMC was significantly decreased in contrast to other analyzed cytokines after contact to the curing CBC compared to control incubations without CBC. This decrease in IL\u20102 release was not due to known inhibitors of IL\u20102 synthesis platelet factor\u20104 (PF\u20104), IL\u201010, TGF\u2010\u03b2, or elevated calcium ion concentrations. \u00a9 2006 Wiley Periodicals, Inc. 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