{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:17:13Z","timestamp":1771237033111,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,5,14]],"date-time":"2007-05-14T00:00:00Z","timestamp":1179100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The synergistic effects of extracellular matrix (ECM) protein combinations on Hep3B cell proliferation and functions are studied herein. Poly(3\u2010hydroxybutyrate\u2010<jats:italic>co<\/jats:italic>\u20103\u2010hydroxyvalerate) (PHBV) microspheres were covalently conjugated with three types of proteins, collagen (type I), laminin, and fibronectin, using 1\u2010ethyl\u20103\u2010(3\u2010dimethylaminopropyl) carbodiimide and <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide cross linkers. Successful conjugations of protein molecules were verified by the presence of nitrogen peaks in X\u2010ray photoelectron spectroscopy. The densities of grafted proteins were quantified using Micro\u2010BCA kit. A human hepatoma cell line, Hep3B, was then cultured <jats:italic>in vitro<\/jats:italic> on the ECM proteins\u2010modified microspheres for 2 weeks. Cell proliferation was estimated using MTT method, and two hepatic functions, albumin secretion and P\u2010450 activity, were evaluated using ELISA and EROD assays, respectively. The results indicated that combination of the three ECM proteins on microsphere surfaces has a significant effect on the proliferation of Hep3B cells, thus better mimicking the <jats:italic>in vivo<\/jats:italic> environment for liver tissue engineering. \u00a9 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007<\/jats:p>","DOI":"10.1002\/jbm.a.31257","type":"journal-article","created":{"date-parts":[[2007,5,14]],"date-time":"2007-05-14T22:48:32Z","timestamp":1179182912000},"page":"606-616","source":"Crossref","is-referenced-by-count":39,"title":["Proteins combination on PHBV microsphere scaffold to regulate Hep3B cells activity and functionality: A model of liver tissue engineering system"],"prefix":"10.1002","volume":"83A","author":[{"given":"Xin Hao","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seng Keat","family":"Gan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Hwa","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen Wah","family":"Tong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,14]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.03.038"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.01.060"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/107632700320883"},{"key":"e_1_2_5_5_2","unstructured":"ClementB LorealO RescanPY LevavasseurF DiakonovaM RisselM L'HelgoualchA GuillouzoA.Cellular origin of the hepatic extracellular matrix. In: Gressner AM Ramador G editors. Molecular and Cell Biology of Liver Fibrinogenesis (Proceedings of the International Falk Symposium Marburg Germany). 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