{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:00:46Z","timestamp":1790863246742,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,4,10]],"date-time":"2008-04-10T00:00:00Z","timestamp":1207785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2009,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>in vitro<\/jats:italic> colonization of three commercial heart valve leaflets by <jats:italic>Staphylococcus aureus<\/jats:italic> was investigated. The leaflets, made of pyrolytic carbon alloyed with or without silicon, displayed similar surface properties (wettability, roughness) and were readily colonized by <jats:italic>S. aureus<\/jats:italic> that formed patchy biofilms on the three supports. A proteomic approach was used to assess the physiological status of biofilm populations by comparing their protein maps to those of bacteria cultured as free cells in the presence or absence of biofilm substratum. Principal component analysis (PCA) revealed, for each tested leaflet, statistical relationships between the protein maps of the biofilm and free\u2010floating microbial populations. A spot\u2010by\u2010spot comparison of protein levels on two\u2010dimensional electropherograms showed that many proteins were accumulated or underproduced by microbial populations grown in the presence of a leaflet compared with protein levels in control free populations. The number of accumulated proteins was noticeably higher than that of underproduced polypeptides. This protein overproduction was emphasized in biofilm populations. Several proteins, some of which were identified, were differentially produced by both surface\u2010associated planktonic and biofilm\u2010grown cell populations compared with control free\u2010cell ones cultured in the absence of leaflet, whatever the leaflet tested. The potential of this proteomic approach for fighting against microbial adhesion and biofilm formation is discussed. \u00a9 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009<\/jats:p>","DOI":"10.1002\/jbm.a.31941","type":"journal-article","created":{"date-parts":[[2008,4,10]],"date-time":"2008-04-10T22:48:08Z","timestamp":1207867688000},"page":"1069-1078","source":"Crossref","is-referenced-by-count":16,"title":["Proteomic analysis of <i>Staphylococcus aureus<\/i> biofilms grown <i>in vitro<\/i> on mechanical heart valve leaflets"],"prefix":"10.1002","volume":"88A","author":[{"given":"Laetitia","family":"B\u00e9nard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre\u2010Yves","family":"Litzler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Cosette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Lemeland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Jouenne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy\u2010Alain","family":"Junter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,4,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra035415"},{"key":"e_1_2_6_3_2","first-page":"340","article-title":"Incidence and risk factors of prosthetic valve endocarditis","volume":"2","author":"Arvay A","year":"1988","journal-title":"Eur J Cardio\u2010Thorac Surg"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199608083350607"},{"key":"e_1_2_6_5_2","first-page":"810","article-title":"Management of infection of major amputation stumps after failed femorodistal grafts","volume":"98","author":"Rubin JR","year":"1985","journal-title":"Surgery"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-5214(05)80009-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.50.1.223"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7944(99)00096-3"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5427-5433.1999"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.003431"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01913-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1008"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.11.008"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2004.06.003"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(03)00123-5"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.6.2770-2780.2002"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.184.4.1140-1154.2002"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200107)1:7<871::AID-PROT871>3.0.CO;2-2"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10937.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11363.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00907-8"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500531"},{"key":"e_1_2_6_23_3","unstructured":"(additional material available on the WWW underhttp:\/\/www.wiley\u2010vch.de\/contents\/jc_2120\/2006\/pro0531_s.pdf)."},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2003.03.001"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00313-6"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surfcoat.2004.02.046"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7765(03)00068-7"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.11.033"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300707"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr034044t"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1980.tb05599.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.41.3.1190-1196.1983"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7765(02)00023-1"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-57105-3_5"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1987.tb02405.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-24-1-65"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7765(98)00037-X"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00494-0"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199610)32:2<249::AID-JBM15>3.0.CO;2-E"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1996.tb03199.x"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:RESB.0000040469.26208.83"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(02)00306-8"},{"key":"e_1_2_6_43_2","first-page":"369","article-title":"Microbiology of the early colonization of human enamel and root surfaces in vivo","volume":"95","author":"Nyvad B","year":"1987","journal-title":"Scand J Dent Res"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1999.tb00396.x"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09249.x"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00204-0"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00542-2"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2004.05.006"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2007.06.013"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr049880x"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03432.x"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-9-2765"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.1.283"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00183-1"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.08.002"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00295-2"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(99)01607-8"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.563-570.2001"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(01)01925-0"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(05)03432-X"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.60.2.439-471.1996"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.11.3636-3642.2005"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.05008.x"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.09.017"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr049880x"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.31941","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.31941","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T16:52:34Z","timestamp":1696870354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.31941"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,4,10]]},"references-count":65,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,3,15]]}},"alternative-id":["10.1002\/jbm.a.31941"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.31941","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,4,10]]}}}