{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T11:47:48Z","timestamp":1723376868397},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T00:00:00Z","timestamp":1229990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2009,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the study, we intend to design a suitable localized drug delivery system (LDDS) with chitosan and poly vinyl alcohol (PVA) for treating serve periodontitis. For that, a novel formulation based on the incorporation of chitosan\u2010based microspheres into PVA film was prepared. As the core parts of the novel formulation, chitosan\u2010based microspheres were prepared form chitosan and\/or carboxymethyl\u2010chitosan (CM\u2010chitosan) by using water\u2010in\u2010oil emulsification method. Then basic <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> experiments focusing on biocompatibility and biodegradability of the two chitosan\u2010based microspheres were carried out to evaluate the feasibility of the novel LDDS. <jats:italic>In vitro<\/jats:italic> tests, besides having no hemolysis, chitosan microsphere (Cs1\u2010Ms), and CM\u2010chitosan microsphere (Cs2\u2010Ms) have adsorbed little proteins on their surfaces. Moreover, plasma proteins adsorbed on Cs2\u2010Ms, most of which can easily desorbed, are much less than that adsorbed on Cs1\u2010Ms. This indicates that Cs2\u2010Ms perhaps has better biocompatibility than Cs1\u2010Ms. <jats:italic>In vivo<\/jats:italic> tests, Cs1\u2010Ms and Cs2\u2010Ms were subcutaneously implanted in rat to investigate the host tissue inflammatory response. Implantations of Cs1\u2010Ms and Cs2\u2010Ms induced a little more severe inflammation when compared with the implantation of PVA film. However, the difference on <jats:italic>in vivo<\/jats:italic> biocompatibility between Cs1\u2010Ms and Cs2\u2010Ms could not be confirmed by the implantation model of our experiments. Both Cs1\u2010Ms and Cs2\u2010Ms had suffered bioerosion when they were subcutaneously implanted. The hard and compact matrixes of Cs1\u2010Ms were degraded very slowly, and only some trifling degradation had been found until 4 weeks of implantation. In contrast, Cs2\u2010Ms is soft and more hydrophilic, and can be quickly degraded in a form of diffluence by the physiological circumstance. All these results suggested that Cs2\u2010Ms had better potentials used as core parts of the novel designed LDDS in the future developments. \u00a9 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009<\/jats:p>","DOI":"10.1002\/jbm.a.32294","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T14:16:42Z","timestamp":1230041802000},"page":"1065-1076","source":"Crossref","is-referenced-by-count":9,"title":["Biological evaluation of a novel chitosan\u2010PVA\u2010based local delivery system for treatment of periodontitis"],"prefix":"10.1002","volume":"91A","author":[{"given":"Ling Chong","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi Guang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"De Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiu Xia","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng Sheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le Jun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Ran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing Sheng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0002-8177(15)30078-7","article-title":"Periodontal diseases: Epidemiology and diagnosis","volume":"129","year":"1998","journal-title":"J Am Dent Assoc"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.2.269"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.02.033"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2006.06.001"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00167-8"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2004.02.002"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.05.043"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2005.08.021"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-007-0159-5"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(01)00502-8"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.10.3819"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118549"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(98)00178-3"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2005.10.019"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(98)80345-X"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2005.07.010"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2003.12.022"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2005.05.038"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.06.036"},{"key":"e_1_2_7_21_2","unstructured":"ISO 10993 Part 4 Selection of tests for interaction with blood. International Standard Organization 1992."},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0376-7388(03)00105-4"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2006.07.008"},{"key":"e_1_2_7_24_2","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1182\/blood.V71.4.932.932","article-title":"Mechanism of transient adsorption of fibrinogen from plasma to solid surfaces: Role of the contact and fibrinolytic systems","volume":"71","author":"Brash JL","year":"1988","journal-title":"Blood"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0939-6411(00)00086-2"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1994.tb00020.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2004.01.019"},{"key":"e_1_2_7_28_2","first-page":"421","article-title":"Principles and clinical applications of periodontal controlled drug delivery with tetracycline fibers","volume":"14","author":"Tonetti MS","year":"1994","journal-title":"Int J Periodontol Res Dent"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(02)00602-6"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-007-3243-y"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.elspec.2004.02.158"},{"key":"e_1_2_7_32_2","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1182\/blood.V74.8.2698.2698","article-title":"Identification of vitronectin as a major plasma protein adsorbed on polymer surfaces of different copolymer composition","volume":"74","author":"Bale MD","year":"1989","journal-title":"Blood"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9797(86)90042-1"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820250106"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.1231"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00469-1"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(96)00167-6"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00094-1"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2003.07.004"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-796X(01)00035-3"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2006.07.025"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(95)99639-4"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0144-8617(01)00320-4"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.32294","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.32294","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T14:55:58Z","timestamp":1696863358000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.32294"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,23]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,12,15]]}},"alternative-id":["10.1002\/jbm.a.32294"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.32294","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,23]]}}}