{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T06:10:17Z","timestamp":1696918217544},"reference-count":68,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,1,22]],"date-time":"2009-01-22T00:00:00Z","timestamp":1232582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2009,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic inflammation may compromise function of implanted encapsulated islets. Increased purity of alginate used for encapsulation prolongs encapsulated graft function, correlating with decreased presence of impurities like bacterial endotoxin. Limits for endotoxin contamination in biomaterials based on indirect inhibition of function of embedded cells have yet to be established. In a coculture system with RAW 264.7 monocyte\/macrophage cells in the presence of 50 ng\/mL murine recombinant gamma\u2010interferon (mrIFN\u2010\u03b3), the insulin response to glucose challenge of both rat and pig unencapsulated islets was prevented by endotoxin (LPS) in the medium down to 0.3 EU\/mL (LOEL), but not 0.06 EU\/mL (NOEL). Evaluation of nitrite concentrations in supernatants revealed that pig islets were more resistant to LPS\u2010stimulated macrophage mediators than rat islets. Encapsulation in highly purified alginate produced little change in observed inhibitory effects of macrophage\u2010generated nitric oxide (NO) toward islet function. Chemically released NO was much less effective in inhibiting insulin responsiveness to glucose challenge than was coculture of islets with LPS and mrIFN\u2010\u03b3\u2010stimulated RAW 264.7. These results taken together with other data suggest that an upper limit of 0.3 EU\/mL LPS within the encapsulating alginate will not impair the function of implanted encapsulated islets by toxic concentrations of macrophage\u2010mediated inflammatory agents. \u00a9 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009<\/jats:p>","DOI":"10.1002\/jbm.a.32351","type":"journal-article","created":{"date-parts":[[2009,1,22]],"date-time":"2009-01-22T13:40:02Z","timestamp":1232631602000},"page":"1221-1238","source":"Crossref","is-referenced-by-count":3,"title":["Sensitivity of insulin production from encapsulated islets to endotoxin\u2010stimulated macrophage inflammatory mediators"],"prefix":"10.1002","volume":"91A","author":[{"given":"Daniel B.","family":"Lyle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan C.","family":"Shallcross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Langone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,22]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/103.4.466"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2003.12.017"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2003.07.008"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(20011205)57:3<374::AID-JBM1180>3.0.CO;2-L"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(01)00503-4"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-003-1087-7"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.1994.tb01467.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.2006.053447"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(96)02051-5"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.46.11.1733"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2004.10.003"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199904150-00008"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(00)02653-1"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368979700600405"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200007270-00025"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00400922"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.3727\/000000004783983738"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/039139889601900408"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.2.311"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.42.3.496"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00399088"},{"key":"e_1_2_6_23_2","first-page":"569","article-title":"Intracellular targets for nitric oxide toxicity to pancreatic \u03b2\u2010cells","volume":"29","author":"Delaney CA","year":"1996","journal-title":"Braz J Med Biol Res"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00605-7"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199311000-00030"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.04.063"},{"key":"e_1_2_6_27_2","unstructured":"ASTMF1903\u201398.Standard practice for testing for biological responses to particles in vitro. In:Annual Book of ASTM Standards Vol13.01.West Conshohocken PA:American Society for Testing Materials;2003. p1003\u20131006."},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199606)31:2<251::AID-JBM12>3.0.CO;2-O"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-005-1359-4"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715760500053651"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1996.d01-728.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32060"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1097\/MPA.0b013e31815ccad2"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1449"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1177\/096368970000900402"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(96)03768-9"},{"key":"e_1_2_6_37_2","first-page":"3220","article-title":"Toxic effects of superoxide, hydrogen peroxide, and nitric oxide on human and pig islets","volume":"27","author":"Jahr H","year":"1995","journal-title":"Transplant Proc"},{"key":"e_1_2_6_38_2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1034\/j.1399-3089.2000.00060.x","article-title":"Inducible nitric oxide synthetase is expressed in adult but not fetal pig pancreatic islets","volume":"7","author":"Feng X","journal-title":"Xenotransplantation"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.3727\/000000003783985142"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000087114.18315.17"},{"key":"e_1_2_6_41_2","first-page":"48","article-title":"Highly efficient isolation of porcine islets of Langerhans for xenotransplantation: Numbers, purity, yield, and in vitro function","volume":"6","author":"Krickhahn M","year":"2001","journal-title":"Ann Transplant"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005340070028"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.127"},{"key":"e_1_2_6_44_2","first-page":"49","article-title":"The dual role of nitric oxide in islet \u03b2\u2010cells","volume":"14","author":"Spinas GA","year":"1999","journal-title":"News Physiol Sci"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00537.2001"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00223.2002"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0801"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4439(93)90144-P"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(94)90093-0"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.12.3450"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.062"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(01)00251-8"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01572.x"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm00115a013"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365519609090591"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0802493"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1288.005"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050298"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.3109\/08916939309043889"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-003-1173-x"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1114\/1.1481054"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050673"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.31452"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-005-0523-2"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568008033340342"},{"key":"e_1_2_6_66_2","first-page":"1","volume-title":"Guideline on Validation of the Limulus Amebocyte Lysate Test as an End\u2010Product Endotoxin Test for Human and Animal Parental Drugs, Biological Products and Medical Devices","author":"US Department of Health and Human Services\/Public Health Services\/Food and Drug Administration","year":"1987"},{"key":"e_1_2_6_67_2","unstructured":"ASTM\u2010F\u20102064\u201300.Standard guide for characterization and testing of alginates as starting materials intended for use in biomedical and tissue\u2010engineered medical products application. In:Annual Book of ASTM Standards Vol.13.01.West Conshohocken PA:American Society for Testing and Materials;2006. p1074\u20131081."},{"key":"e_1_2_6_68_2","doi-asserted-by":"publisher","DOI":"10.1159\/000014419"},{"key":"e_1_2_6_69_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0894-0959.2004.17617.x"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.32351","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.32351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T14:56:59Z","timestamp":1696863419000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.32351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,22]]},"references-count":68,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,12,15]]}},"alternative-id":["10.1002\/jbm.a.32351"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.32351","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,22]]}}}