{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:01:39Z","timestamp":1761058899934},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,20]],"date-time":"2009-02-20T00:00:00Z","timestamp":1235088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cr (VI) is a well\u2010recognized environmental toxin and carcinogen. It is known to be released from orthopedic metal implants <jats:italic>in\u2010situ<\/jats:italic> by biocorrosion and is speculated to play a role in periprosthetic osteolysis. It is hence essential to understand its long\u2010term biological effects. We have assessed the <jats:italic>in vitro<\/jats:italic> responses of osteoblasts and monocytes to chronic exposure (3 weeks) to Cr (VI), at concentrations that have been measured in patients with metal implants, using two\u2010dimensional gel electrophoresis. Cr (VI) exposure resulted in a differential time\u2010dependent regulation of glycolytic, stress, and cytoskeletal proteins. The proteins that have been found to be altered in expression play an essential role in normal cellular functioning such as energy metabolism, cell signaling, and proliferation. The results highlight the complex molecular changes that occur in both cell types with long\u2010term exposure to Cr and may be useful in establishing a series of clinically useful biomarkers to monitor long\u2010term use of metallic implants. \u00a9 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010<\/jats:p>","DOI":"10.1002\/jbm.a.32396","type":"journal-article","created":{"date-parts":[[2009,2,20]],"date-time":"2009-02-20T16:33:23Z","timestamp":1235147603000},"page":"615-625","source":"Crossref","is-referenced-by-count":12,"title":["Changes in protein expression associated with chronic <i>in vitro<\/i> exposure of hexavalent chromium to osteoblasts and monocytes: A proteomic approach"],"prefix":"10.1002","volume":"92A","author":[{"given":"Vijay Krishna","family":"Raghunathan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Helen","family":"Grant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth M.","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,20]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matdes.2005.05.018"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820290510"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(02)00378-5"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.04.002"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2005.04.005"},{"key":"e_1_2_7_7_2","first-page":"46","article-title":"Analysis of 118 second generation metal on metal retrieved hip implants","volume":"80","author":"Seiber HP","year":"1998","journal-title":"J Bone Joint Surg Br"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00041-7"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/109374099281241"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:MCBI.0000007255.72746.a6"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2003.12.014"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20710"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2005.03.015"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.3.C868"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.03.012"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(03)00059-5"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(03)00471-4"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(99)00075-2"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1013630401959"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017974415052"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31893"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018510900941"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2003.08.014"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2002.53"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/315688a0"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052592699"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3440837"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012432399"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(97)00087-8"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/1040-8428(94)00149-N"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-006-9146-2"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(95)00233-2"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.83B7.10102"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115768"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509497200"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2006.02.021"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507124200"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(01)00289-7"},{"key":"e_1_2_7_40_2","doi-asserted-by":"crossref","first-page":"10312","DOI":"10.1016\/S0021-9258(17)44459-0","article-title":"Distinct patterns of cytoplasmic protein phophorylation related to regulation of synthesis and release of prolactin by GH cells","volume":"258","author":"Sobel A","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.22.3333"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.tb20401.x"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19991215)47:4<543::AID-JBM11>3.0.CO;2-G"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.32396","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.32396","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T12:44:31Z","timestamp":1697460271000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.32396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,20]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1002\/jbm.a.32396"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.32396","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2,20]]}}}