{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T09:26:37Z","timestamp":1782725197127,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,6,30]],"date-time":"2009-06-30T00:00:00Z","timestamp":1246320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study presents a new approach for evaluating <jats:italic>in vitro<\/jats:italic> cytotoxicity of nanoparticles. The approach is based on American National Standard ISO 10993\u20105. Hepatoma HepG2 and fibroblast NIH3T3 cell lines were incubated with nanoparticles, and their associated extracts were derived at 70 and 121\u00b0C. Nanoparticles proposed as potential biomedical imaging probes were evaluated on the basis of the detection of metabolic activities and cell\u2010morphology changes. In general, nanoparticles incubated directly with cells showed higher cytotoxicity than their associated extracts. CdSe and core\u2010shell CdSe@ZnS quantum dots resulted in low cell viability for both cell lines. The cytotoxicity of the quantum dots was attributed to the Cd ion and the presence of the nanoparticle itself. A statistically significant (<jats:italic>p<\/jats:italic> &lt; 0.05) decrease in cell viability was found in higher dosage concentrations. Rare earth nanoparticles and their extracts appear to affect NIH3T3 cells only, with cell viability as low as 71.4% \u00b1 4.8%. Magnetic nanoparticles have no observable effects on the cell viabilities for both cell lines. In summary, we found the following: (1) both direct incubation and extracts of nanoparticles are required for complete assessment of nanoparticle cytotoxicity, (2) the rare earth oxide nanoparticles are less cytotoxic than the Cd\u2010based quantum dots, and (3) the extent of cytotoxicity is dependent upon the cell line. \u00a9 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010<\/jats:p>","DOI":"10.1002\/jbm.a.32533","type":"journal-article","created":{"date-parts":[[2009,6,30]],"date-time":"2009-06-30T18:25:49Z","timestamp":1246386349000},"page":"337-346","source":"Crossref","is-referenced-by-count":42,"title":["<i>In vitro<\/i> cytotoxicity evaluation of biomedical nanoparticles and their extracts"],"prefix":"10.1002","volume":"93A","author":[{"given":"Gautom Kumar","family":"Das","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy P. 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