{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:35:42Z","timestamp":1762673742834},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T00:00:00Z","timestamp":1266796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polymethylmethacrylate (PMMA) particles have been shown to inhibit the differentiation, proliferation, and mineralization of osteoprogenitor cells <jats:italic>in vitro<\/jats:italic>. In this study, we investigated the effects of OP\u20101 (BMP\u20107) on the osteogenesis of MC3T3\u2010E1 osteoprogenitor cells exposed to PMMA particles <jats:italic>in vitro<\/jats:italic>. MC3T3\u2010E1 cells challenged with PMMA particles on the 1st day of differentiation in osteogenic culture showed a significant dose\u2010dependent decrease in mineralization and alkaline phosphatase expression over a 20\u2010day culture period. Exposure of these cells to OP\u20101 (200 ng\/mL) during days 1\u20134, 1\u201320, and 4\u201320 in the presence of PMMA particles resulted in significant increases in mineralization and alkaline phosphatase expression at all particle doses. Addition of OP\u20101 to MC3T3\u2010E1 cultures challenged with PMMA particles on the 4th day of differentiation in osteogenic media also resulted in significant increases in mineralization and alkaline phosphatase expression. This study has shown that OP\u20101 stimulates osteogenesis in MC3T3\u2010E1 osteoprogenitor cells that have been inhibited by PMMA particles. Local administration of OP\u20101 to the site of osteolysis may be a potential adjunctive therapy to reverse the bone destruction due to wear particles. \u00a9 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010<\/jats:p>","DOI":"10.1002\/jbm.a.32712","type":"journal-article","created":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T19:52:19Z","timestamp":1266868339000},"page":"485-488","source":"Crossref","is-referenced-by-count":13,"title":["OP\u20101 (BMP\u20107) stimulates osteoprogenitor cell differentiation in the presence of polymethylmethacrylate particles"],"prefix":"10.1002","volume":"94A","author":[{"given":"Shawn","family":"Kann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart B.","family":"Goodman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,2,22]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Implant Wear in Total Joint Replacement","author":"Wright T","year":"2001"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20618"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(02)00076-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20075"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012470862-4\/50003-9"},{"key":"e_1_2_6_7_2","unstructured":"MckeeMD SchemitschEH WaddelJP HansJK StephenDJ LeightonRK BuckleyRE PowellJN WildLM BlachutPA O'BrienPJ PiraniS McCormackRG. The effect of human recombinant bone morphogenic protein (RHBMP\u20107) on the healing of open tibial shaft fractures: Results of a multi\u2010center prospective randomized clinical trial. In:Proceedings of the 18th Annual Meeting of the Orthopaedic Trauma Association Toronto 2002. p157\u2013158."},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.89B2.18230"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000216444.01888.21"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1529-9430(02)00180-8"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-003-0561-8"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000137062.79201.98"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-004-0845-7"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.32712","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.32712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T15:15:02Z","timestamp":1696864502000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.32712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,22]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1002\/jbm.a.32712"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.32712","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,22]]}}}