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In liver therapeutic research, hepatocytes require attachment to solid anchors for survival and proliferation before they could grow into cellular aggregates with enhanced functionalities. Among the various biomaterials scaffolds and vehicles, microspherical cell carriers are suited to these requirements. Individual spheres may provide two\u2010dimensional (2D) cell\u2010affinitive surfaces for cell adhesion and spreading; whereas multiple microcarriers may form three\u2010dimensional (3D) matrices with inter\u2010spherical space for cell expansion and multicellular aggregation. In this study, we culture human liver carcinoma cell line (HepG2) cells on genipin\u2010crosslinked gelatin microspheres of two different sizes. Results suggest that both microcarriers support cell adhesion, proliferation, and spontaneous formation of hepatocellular aggregates, among which the spheres with bigger size (200\u2013300 \u03bcm) seem more favorable than the smaller ones in terms of aggregate formation and liver specific functionalities. These findings suggest that the genipin\u2010crosslinked microcarrier is a competent vehicle for liver cell delivery. \u00a9 2010 Wiley Periodicals, Inc. 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