{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:27:56Z","timestamp":1759177676659},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T00:00:00Z","timestamp":1304467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to evaluate the <jats:italic>in vitro<\/jats:italic> biocompatibility of the novel Sparfoxacin (SPA)\u2010treated latex catheter with previously performed prolonged antimicrobial activity. Rectangular\u2010shaped test samples of silicone latex catheter were fabricated according to patented procedure permitting the immobilization of SPA on heparin (HP)\u2010coated catheter by means of mixed, covalent and non\u2010covalent bonds. Samples subjected to cytotoxicity assay were divided into four groups: (1) the untreated catheter, (2) HP\u2010coated catheter, (3) HP\u2010coated catheter with SPA immobilized in low SPA concentration solution (SPA\u2010L treated sample), and (4) high SPA concentration solution (SPA\u2010H treated sample). Then the samples were placed directly into green monkey kidney (GMK) cell monolayer for 24 h. After the incubation period, cytotoxicity was determined by 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102H\u2010tetrazolium bromide (MTT) assay. The degree of cytotoxicity of each sample was evaluated according to the reference value represented by the control cells cultured without catheter sample. Statistical significance was determined by repeated <jats:italic>t<\/jats:italic>\u2010test (<jats:italic>P<\/jats:italic> &lt; 0.05). The cytotoxic effect of treated and untreated catheters was also estimated by microscopic observations of GMK cells morphological changes. SPA\u2010treated catheters demonstrated high survival rates in MTT assay (&gt;93%) on the contrary to the untreated catheters (6.13%) and HP\u2010coated catheters (80.90%). Moreover, microscopic observation of GMK cells exposed to SPA\u2010treated samples revealed no morphological changes and no cell growth reduction. We suggest that SPA\u2010treated latex catheters are biofilm formation resistant (as we revealed in our previous work), considerably less toxic than untreated ones, and can be undoubtedly used in urological practice. \u00a9 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.<\/jats:p>","DOI":"10.1002\/jbm.a.33110","type":"journal-article","created":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T15:44:49Z","timestamp":1304523889000},"page":"222-228","source":"Crossref","is-referenced-by-count":15,"title":["The cytotoxicity assessment of the novel latex urinary catheter with prolonged antimicrobial activity"],"prefix":"10.1002","volume":"98A","author":[{"given":"Dorota","family":"Kowalczuk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazyna","family":"Ginalska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Przekora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,5,4]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"296","article-title":"Ureteral stents: Use and complications","volume":"6","author":"Mardis HK","year":"1992","journal-title":"Probl Urol"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820180914"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1080\/21681805.1991.12068896","article-title":"Biocompatibility of urinary catheters\u2014Present status","volume":"138","author":"Ruutu M","year":"1991","journal-title":"Scand J Urol Nephrol Suppl"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005392-199502000-00069"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(98)00198-8"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00368190"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1985.tb08992.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1985.tb05870.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000020346"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1991.tb15143.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2006.01.020"},{"key":"e_1_2_6_13_2","unstructured":"GinalskaG KowalczukDinventors. 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