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Four new conduits were, therefore, obtained and named by immobilizing order\u2010EDCs\/EDCs, GP\/EDCs, EDCs\/GP, and GP\/GP groups. The immobilized methods used were evaluated and compared, respectively. The researchers found that the EDCs\u2010 and GP\u2010cross\u2010linked chitosan displayed higher hydrophilic than pure poly (<jats:sc>DL<\/jats:sc>\u2010lactic acid\u2010co\u2010glycolic acid) (PLGA) in water contact angle experiment, which meant the cell compatibility was improved by the modification. Scanning electron microscopic observations revealed that the GP\u2010cross\u2010linking of chitosan greatly improved cell compatibility while cultured rat PC12 cells were flatter and more spindle\u2010shaped than EDCs\u2010cross\u2010linked chitosan. The results concerning the GP\u2010cross\u2010linked chitosan revealed significant proliferation of the seeded cells relative to pure PLGA films, as determined by counting cells and MTT assay. The NGF was released from the modified conduits in two separate periods\u2014an initial burst in 5 days and then slow release from day 10 to day 40. The GP\/EDCs group had the highest NGF value among all groups after the 5th day. Finally, the controlled\u2010release conduits were used to bridge a 10 mm rat sciatic nerve defect. Six weeks following implantation, morphological analysis revealed the highest numbers of myelinated axons in the midconduit and distal regenerated nerve in GP\/EDCs group. Therefore, the results confirm that GP\/EDCs groups with good cell compatibility and effective release of NGF can considerably improve peripheral nerve regeneration. \u00a9 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.<\/jats:p>","DOI":"10.1002\/jbm.a.33157","type":"journal-article","created":{"date-parts":[[2011,9,28]],"date-time":"2011-09-28T02:27:54Z","timestamp":1317176874000},"page":"576-585","source":"Crossref","is-referenced-by-count":16,"title":["Comparison between two different methods of immobilizing NGF in poly(<scp>DL<\/scp>\u2010lactic acid\u2010co\u2010glycolic acid) conduit for peripheral nerve regeneration by EDC\/NHS\/MES and 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