{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:19:21Z","timestamp":1790813961798,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T00:00:00Z","timestamp":1332460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bone\u2013implant interface is critical for the early fixation of orthopedic implants. In this study, porous hydroxyapatite (HA) coatings were prepared through a liquid precursor plasma spraying process and were infiltrated with the collagen, alone and with the additional incorporation of recombinant human bone morphogenetic protein\u20102 (rhBMP\u20102) and RGD peptide (RGD). The results showed significantly improved mesenchymal stem cell (MSC) adhesion, proliferation, and differentiation on collagen\u2010modified HA coatings, partially benefited from the formation of a fibrous network due to the self\u2010reconstitution of collagen on the HA surface. Further enhancements on MSC proliferation and differentiation were generally observed through the additional incorporation of bone morphogenetic protein (BMP) and RGD. The osteoinductive and osteoconductive properties of the collagen\/BMP\u2010modified HA coatings were studied <jats:italic>in vivo<\/jats:italic>. Clear ectopic bone formation and significantly accelerated bone growth rate (29% increase, <jats:italic>p<\/jats:italic> &lt; 0.05) have been observed after 1\u2010month implantation of HA\u2013collagen\/rhBMP\u20102\u2010coated Ti alloy samples into the rabbit muscle and dog femora, respectively. Overall, our results suggest that collagen\u2010modified HA coating surface is a far superior substrate for cell attachment, proliferation, and differentiation, and collagen can be used an efficient carrier for BMP <jats:italic>in vivo<\/jats:italic>. Therefore, modification of HA coating with collagen is a simple but effective biomimetic approach to enhancing the osteointegration and early fixation of bone\u2013implant interface. \u00a9 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.<\/jats:p>","DOI":"10.1002\/jbm.a.34121","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T17:18:28Z","timestamp":1332523108000},"page":"1706-1715","source":"Crossref","is-referenced-by-count":60,"title":["Collagen\u2010infiltrated porous hydroxyapatite coating and its osteogenic properties: <i>In vitro<\/i> and <i>in vivo<\/i> 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