{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:23Z","timestamp":1786977863594,"version":"build-2736575974"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,7,20]],"date-time":"2012-07-20T00:00:00Z","timestamp":1342742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Amputation places a significant burden on healthcare systems worldwide as patients suffer life\u2010long complications associated with the stump\u2010socket interface. Skin penetrating, osseointegrated implants like intraosseous transcutaneous amputation prostheses, could overcome this, however, they rely on the formation and maintenance of an infection\u2010free seal at the skin\u2010implant interface. Epithelial cell migration around transcutaneous implants creates downgrowth, which leads to infection and implant failure. Epithelial cells form cell\u2013cell attachments via adherens junctions and desmosomes that prevent cell migration via contact inhibition. If epithelial cells formed cell\u2013cell attachments with an implant surface, it could facilitate stronger cell attachment and prevent downgrowth. In adherens junctions, E\u2010cadherin is essential in homotypic cell attachment. In this study, we have demonstrated that cell\u2013cell adherens junctions can be formed on substrates adsorbed with E\u2010cadherin. We have assessed the effects of two E\u2010cadherin peptides and determined an optimal concentration for increasing cell attachment via adherens junctions. We have demonstrated that adsorption of 15 \u03bcg\/mL of the full extracellular domain of E\u2010cadherin to titanium alloy significantly increases metabolic activity, cell area, and attachment of murine keratinocytes\n                    <jats:italic>in vitro<\/jats:italic>\n                    , with a fourfold increase in attachment via adherens junctions at 24, 48, and 72 h. \u00a9 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3463\u20133471, 2012.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.34290","type":"journal-article","created":{"date-parts":[[2012,7,20]],"date-time":"2012-07-20T19:22:29Z","timestamp":1342812149000},"page":"3463-3471","source":"Crossref","is-referenced-by-count":17,"title":["The effect of adherens junction components on keratinocyte adhesion\n                    <i>in vitro<\/i>\n                    : Potential implications for sealing the skin\u2010implant interface of intraosseous transcutaneous amputation prostheses"],"prefix":"10.1002","volume":"100A","author":[{"given":"Catherine Jane","family":"Pendegrass","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethan","family":"Tucker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelain","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Dowling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon William","family":"Blunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.2000.01344.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/15419069809069760"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.4.589"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8281-1"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(00)00231-8"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.3.1047"},{"key":"e_1_2_6_8_2","first-page":"1028","article-title":"Measuring the strength of dermal fibroblast attachment to functionalised titanium alloys in vitro","volume":"92","author":"Pendegrass C","year":"2009","journal-title":"J Biomed Mater Res A"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/02713689508995820"},{"key":"e_1_2_6_10_2","article-title":"Mouse out root sheath cells cultured on E\u2010cadherin\u2010coated dishes exhibit neuronal cell morphology","author":"Lingling X","journal-title":"Asia\u2010Pac J Chem Eng."},{"key":"e_1_2_6_11_2","unstructured":"DavisU. 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Available at:http:\/\/www.mcb\/ucdavis.edu?courses\/bis102\/AAProp.html."},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1002\/jbm.a.32735","article-title":"Modification of titanium alloy surfaces for percutaneous implants by covalently attaching laminin","volume":"94","author":"Gordon D","year":"2010","journal-title":"J Biomed Mater Res A"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1008"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.070052697"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.34290","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.34290","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.a.34290","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.34290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T05:39:55Z","timestamp":1714196395000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.34290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,20]]},"references-count":13,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1002\/jbm.a.34290"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.34290","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,20]]}}}