{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:26:20Z","timestamp":1761675980529},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T00:00:00Z","timestamp":1343692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study is to investigate <jats:italic>in vitro<\/jats:italic> release and cell response to wear particles of ultrahigh molecular weight polyethylene loaded with alendronate sodium (UHMWPE\u2010ALN), a potent bone resorption inhibitor. Wear particles of UHMWPE\u2010ALN with different ALN contents (0.5 wt % or 1.0 wt %) and size ranges (&lt;45 \u03bcm or 45\u201375 \u03bcm) were cocultured with macrophages (RAW264.7) and osteoblasts (MC3T3\u2010E1), respectively. The <jats:italic>in vitro<\/jats:italic> ALN release was divided into three stages: an initial burst release, subsequent rapid release, and final slow release. The particle size and ALN content of UHMWPE\u2010ALN wear particles affected the <jats:italic>in vitro<\/jats:italic> release mainly during initial burst and rapid release. Compared with the control cells, UHMWPE\u2010ALN wear particles stimulated a significant elevation of tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) release from macrophages but had no obvious effect on interleukin\u20106 release. However, this stimulation of TNF\u2010\u03b1 release could be reduced by ALN released from UHMWPE\u2010ALN wear particles. The wear particle size had stronger effect of on the macrophages compared with the ALN concentration. After coculture with UHMWPE\u2010ALN wear particles, osteoblast proliferation and alkaline phosphatase activities increased moderately with the increase in particle sizes and ALN concentrations. These results suggest that incorporation of ALN in UHMWPE\u2010ALN may be an effective approach to prevent or reduce particles\u2010induced osteolysis. \u00a9 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.<\/jats:p>","DOI":"10.1002\/jbm.a.34327","type":"journal-article","created":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T07:13:59Z","timestamp":1343718839000},"page":"394-403","source":"Crossref","is-referenced-by-count":25,"title":["Study on <i>in vitro<\/i> release and cell response to alendronate sodium\u2010loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles\u2010induced osteolysis"],"prefix":"10.1002","volume":"101A","author":[{"given":"Shuxin","family":"Qu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinlong","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Weng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1520\/STP1445-EB"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60457-7"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.engfailanal.2006.11.005"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1097\/00003086-199203000-00003","article-title":"Mechanism and clinical significance of wear particles\u2010induced osteolysis","volume":"276","author":"Amstutz HC","year":"1992","journal-title":"Clin Orthop Res"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matdes.2003.11.010"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1097\/00003086-199009000-00013","article-title":"Osteolysis in all arthroplasty of the hip. The role of ultra\u2010high molecular weight polyethylene wear particles","volume":"258","author":"Willert HG","year":"1990","journal-title":"Clin Orthop Relat Res"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(20000605)50:3<381::AID-JBM12>3.0.CO;2-P"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vacuum.2003.12.160"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.12.048"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2004.09.011"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(01)00069-5"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2006.09.031"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2008.08.005"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.G.00415"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.10.040"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/4\/6\/065008"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2011.09.012"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(98)00140-9"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820280111"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.51.1444"},{"key":"e_1_2_6_22_2","volume-title":"ASTM F 2102\u201306, Standard Guide for Evaluating the Extent of Oxidation in Ultra\u2010High\u2010Molecular\u2010Weight Polyethylene Fabricated Forms Intended for Surgical Implants"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00033-3"},{"key":"e_1_2_6_24_2","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1002\/jbm.a.32803","article-title":"Effects of orthopedic polymer particles on chemotaxis of macrophages and mesenchymal stem cells","volume":"94","author":"Huang ZN","year":"2010","journal-title":"J Biomed Mater Res A"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbbm.2006.05.011"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10455"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1648(01)00589-0"},{"key":"e_1_2_6_28_2","first-page":"189","article-title":"Quantitative analysis of UHMWPE wear de particles isolated from the periprosthetic femoral tissues from a series of Charnley total hip arthroplasties","volume":"212","author":"Bell J","year":"2000","journal-title":"Bio Med Mater Eng"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(02)00136-0"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11249-009-9563-y"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.wear.2010.11.048"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2006.11.007"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(01)00147-9"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.054"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.08.023"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-3278(97)90508-1"},{"key":"e_1_2_6_37_2","first-page":"6001","article-title":"Bisphosphonates directly regulate cell proliferation, differentiation, and gene expression in human osteoblasts","volume":"60","author":"Reinholz GG","year":"2000","journal-title":"Cancer Res"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.34327","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.34327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T01:32:32Z","timestamp":1696901552000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.34327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,31]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1002\/jbm.a.34327"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.34327","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,31]]}}}