{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:51:23Z","timestamp":1792457483472,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T00:00:00Z","timestamp":1358985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fluorescent silica nanoparticles are reported to be highly stable and biocompatible materials with high water solubility, which make them ideal candidates for biological applications. These nanoparticles can also be modified with biocompatible and targeting moieties and can be used for a variety of <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> applications, such as targeting, particle tracking, cargo carrier, and as contrast agents. In this study, fluorescent dye\u2010doped silica nanoparticles were prepared by a modified St\u00f6ber method. The nanoparticles produced were surface functionalized with amine moieties for their conjugation with glucose\u2010derived and galactose\u2010based residues. The amine, glucose\u2010derived, and galactose\u2010based functionalized fluorescent silica nanoparticles were analyzed for their physiochemical properties such as sizes, polydispersities, organic layer content, and surface chemistries. The nanoparticles produced were then studied for their interactions with carbohydrate\u2010specific lectins. These lectin bioconjugates have helped in understanding their interactions with cell\u2010surface receptors. As expected, galactose\u2010functionalized nanoparticles were found to specifically interact with RCA<jats:sub>120<\/jats:sub>, as compared to other nanoparticles. These specific interactions of galactose\u2010lectin conjugates were further studied on the hepatocytes cell surface <jats:italic>in vitro<\/jats:italic>. The aggregation of galactose\u2010lectins conjugates on the plasma membrane was possibly due to the specific interactions of carbohydrates with cell\u2010surface glycoproteins, hence preventing the uptake of these nanoparticles. The study has provided an interesting approach to mark the cell\u2010surface glycoproteins with fluorescent probes using a combination of lectin\u2010carbohydrate conjugates. \u00a9 2013 Wiley Periodicals, Inc. 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