{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:15:56Z","timestamp":1769732156644,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T00:00:00Z","timestamp":1380499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The periprosthetic osteolysis induced by wear particles contributes to aseptic loosening after joint arthroplasty. The molecular mechanism underlying osteolysis remains to be described. In this study, cultured MC3T3\u2010E1 cells were incubated with titanium particles. We investigated the role of p38 mitogen\u2010activated protein kinase in the expression of MMP\u20102 in response to wear particles. Our results demonstrated MC3T3\u2010E1 cells exposed to titanium particles had significantly increased levels of MMP\u20102 and MT1\u2010MMP mRNA, whereas the TIMP\u20102 mRNA level was unchanged. In MC3T3\u2010E1 cells, the protein expression of MMP\u20102, MT1\u2010MMP, and active p38 was also elevated after titanium particle exposure, as detected by Western blot and Biotrak activity analyses. Inhibition studies showed that the specific p38 inhibitor SB203580 completely abrogated the increase in MMP\u20102 and MT1\u2010MMP production induced by the titanium particles. Moreover, our results revealed that conditioned media\u2010stimulated osteoclast formation was related to the MMP\u20102 activity of osteoblasts that were challenged with Ti particles. This study demonstrated that p38 signaling is required for MMP\u20102 activity in osteoblasts under wear particle\u2010induced conditions. MMP\u20102 could act as a catabolic element or a proinflammatory factor contributing to periprosthetic osteolysis. Therefore, the p38 pathway and MMP\u20102 may play a critical role in the development of aseptic loosening. \u00a9 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2824\u20132832, 2014.<\/jats:p>","DOI":"10.1002\/jbm.a.34956","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T21:58:24Z","timestamp":1381528704000},"page":"2824-2832","source":"Crossref","is-referenced-by-count":21,"title":["p38 signaling in titanium particle\u2010induced MMP\u20102 secretion and activation in differentiating MC3T3\u2010E1 cells"],"prefix":"10.1002","volume":"102","author":[{"given":"Ming","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopedics Second Affiliated Hospital of Soochow University 1055, Sanxiang Road Suzhou 215004, Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Second Affiliated Hospital of Soochow University 1055, Sanxiang Road Suzhou 215004, Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Rong","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Second Affiliated Hospital of Soochow University 1055, Sanxiang Road Suzhou 215004, Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Second Affiliated Hospital of Soochow University 1055, Sanxiang Road Suzhou 215004, Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"She","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Second Affiliated Hospital of Soochow University 1055, Sanxiang Road Suzhou 215004, Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Second Affiliated Hospital of Soochow University 1055, Sanxiang Road Suzhou 215004, Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(02)00241-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31996"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.03.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000150451.50452.da"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp2000555"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000238813.95035.1b"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2009.05.020"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.08.027"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2741\/2024"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2170"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.5872"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(97)90025-1"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.1.77"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2005.06.009"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-051060"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.2.288"},{"key":"e_1_2_6_18_1","first-page":"677","article-title":"Inhibitory effect of curcumin on MMP\u20102 and MMP\u20109 expression induced by polyethylene wear particles and its mechanism","volume":"23","author":"Qi Q","year":"2009","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500693102"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/jbm.a.32001","article-title":"Ultrahigh molecular weight polyethylene wear debris inhibits osteoprogenitor proliferation and differentiation in vitro","volume":"89","author":"Chiu R","year":"2009","journal-title":"J Biomed Mater Res A"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.E.00711"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-012-9500-3"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.33197"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orthres.2003.08.013"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.12.018"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23863"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22002"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21458"},{"key":"e_1_2_6_29_1","first-page":"1055","article-title":"The potential role of human osteoblasts for periprosthetic osteolysis following exposure to wear particles","volume":"28","author":"Lochner K","year":"2011","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.11.2043"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4682-9-33"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.17.4782"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0086"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100323"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(03)00081-0"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32686"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2009.06.014"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34122"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.03.010"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32836"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1402.027"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.34956","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.34956","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T16:35:41Z","timestamp":1716050141000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.34956"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,30]]},"references-count":40,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1002\/jbm.a.34956"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.34956","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,30]]}}}