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Long\u2010term antibacterial properties and the ability to inhibit biofilm formation are highly desirable to prevent implant associated infection. In this study, a controllable amount of silver (Ag) nanoparticles was incorporated into titanium oxide; or titanium, nanotubes (TiO<jats:sub>2<\/jats:sub>\u2010NTs). The reliable release and long\u2010term antibacterial function of Ag, <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>, and influence normal bone\u2010implant integration from the Ag released from Ag\u2010incorporated NTs <jats:italic>in vivo<\/jats:italic> have been studied to make them useable in clinical practice. In the current study, TiO<jats:sub>2<\/jats:sub>\u2010NTs loaded with Ag (NT\u2010Ag) exhibited strong antibacterial activity against methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA, ATCC43300) <jats:italic>in vitro<\/jats:italic> for 30 days, and the ability to penetrate the protein layer well. In addition, X\u2010ray examination and 2\u2010[<jats:sup>18<\/jats:sup>F]\u2010fiuoro\u20102\u2010deoxy\u2010D\u2010glucose positron emission tomography indicates that NT\u2010Ag show extremely long antibacterial activity <jats:italic>in vivo<\/jats:italic> in a rat model. Furthermore, histomorphometric analysis demonstrated that satisfactory bio\u2010integration can be expected. Our results indicate that NT\u2010Ag has both simultaneous antimicrobial and excellent bio\u2010integration properties, make it a promising therapeutic material for orthopedic application. \u00a9 2013 Wiley Periodicals, Inc. 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