{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:22:27Z","timestamp":1789179747761,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T00:00:00Z","timestamp":1405382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Technologies R&D Program","award":["2012BAI17B02"],"award-info":[{"award-number":["2012BAI17B02"]}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["51273072"],"award-info":[{"award-number":["51273072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["50803018"],"award-info":[{"award-number":["50803018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["51232002"],"award-info":[{"award-number":["51232002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003453","name":"Nature Science Foundation of Guangdong","doi-asserted-by":"crossref","award":["2012A080203010"],"award-info":[{"award-number":["2012A080203010"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003453","name":"Nature Science Foundation of Guangdong","doi-asserted-by":"crossref","award":["2012A080800015"],"award-info":[{"award-number":["2012A080800015"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although collagen with outstanding biocompatibility has promising application in corneal tissue engineering, the mechanical properties of collagen\u2010based scaffolds, especially suture retention strength, must be further improved to satisfy the requirements of clinical applications. This article describes a toughness reinforced collagen\u2010based membrane using silk fibroin. The collagen\u2010silk fibroin membranes based on collagen [silk fibroin (w\/w) ratios of 100:5, 100:10, and 100:20] were prepared by using silk fibroin and cross\u2010linking by 1\u2010ethyl\u20103\u2010(3\u2010dimethylaminopropyl) carbodiimide. These membranes were analyzed by scanning electron microscopy and their optical property, and NaCl and tryptophan diffusivity had been tested. The water content was found to be dependent on the content of silk fibroin, and CS10 membrane (loading 10 wt % of silk fibroin) performed the optimal mechanical properties. Also the suture experiments have proved CS10 has high suture retention strength, which can be sutured in rabbit eyes integrally. Moreover, the composite membrane proved good biocompatibility for the proliferation of human corneal epithelial cells <jats:italic>in vitro<\/jats:italic>. Lamellar keratoplasty shows that CS10 membrane promoted complete epithelialization in 35 \u00b1 5 days, and their transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization, and keratoconus are not observed. The composite films show potential for use in the field of corneal tissue engineering. \u00a9 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1159\u20131168, 2015.<\/jats:p>","DOI":"10.1002\/jbm.a.35268","type":"journal-article","created":{"date-parts":[[2014,7,7]],"date-time":"2014-07-07T08:24:48Z","timestamp":1404721488000},"page":"1159-1168","source":"Crossref","is-referenced-by-count":93,"title":["Improving the mechanical properties of collagen\u2010based membranes using silk fibroin for corneal tissue engineering"],"prefix":"10.1002","volume":"103","author":[{"given":"Kai","family":"Long","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weichang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichong","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhongshan Ophthalmic Center Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Materials Science and Engineering South China University of Technology Guangzhou China"},{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-432-6_2"},{"key":"e_1_2_6_3_1","volume-title":"Prevention of Blindness and Visual Impairment: Priority Eye Diseases","author":"WHO","year":"2013"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.126.10.1457"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.08.033"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-432-6_10"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.04.014"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.07.027"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.11.022"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.11.011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-11799"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e318264ee6a."},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.041"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.11.011"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/la803736x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.06.017"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.09.026"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34317"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2011.08.021"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34710"},{"key":"e_1_2_6_21_1","first-page":"545","article-title":"Biocompatibility evaluation of electrospun silk fibroin nanofibrous mats with primarily cultured rat hippocampal neurons","volume":"23","author":"Zhao Y","year":"2013","journal-title":"Biomed Mater Eng"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065654"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.12.155"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201200097"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.01.016"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.12.034"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.03.068"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1163\/092050610X538218"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2012.08.030"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2013.07.009"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/1\/4\/010"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2013.06.050"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.14.004662"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.35268","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.35268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T19:08:58Z","timestamp":1697742538000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.35268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,15]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1002\/jbm.a.35268"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.35268","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,15]]}}}