{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T02:34:06Z","timestamp":1789958046566,"version":"4.0.1"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T00:00:00Z","timestamp":1411516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Stem Cell Technology Research Center"},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NSC","award":["103-2221-E-011-032"],"award-info":[{"award-number":["103-2221-E-011-032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Differentiation of mesenchymal stem cells (MSCs) into neuron cells has great potential in therapy of damaged nerve tissue. It has been shown that three\u2010dimensional biomaterials have great ability to up regulate the expression of neuronal proteins. In this study, O<jats:sub>2<\/jats:sub>plasma technology was used to enhance hydrophilicity of poly (<jats:italic>\u03b5<\/jats:italic>\u2010caprolactone) (PCL) toward selective differentiation of MSCs into neural cells. Random and aligned PCL nanofibers scaffolds were fabricated by electrospinning method and their physicochemical and mechanical properties were carried out by scanning electron microscope (SEM), contact angle, and tensile measurements. Contact angle studies of PCL and plasma treated PCL (<jats:italic>p<\/jats:italic>\u2010PCL) nanofibers revealed significant change on the surface properties PCL nanofibers from the view point of hydrophilicity. Physiochemical studies revealed that<jats:italic>p<\/jats:italic>\u2010PCL nanofibers were extremely hydrophilic compared with untreated PCL nanofibers which were highly hydrophobic and nonabsorbent to water. Differentiation of MSCs were carried out by inducing growth factors including basic fibroblast growth factor, nerve growth factor, and brain derived growth factor, NT3, 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX) in Dulbecco's modified Eagle's medium\/F12 media. Differentiated MSCs on nanofibrous scaffold were examined by immunofluorescence assay and was found to express the neuronal proteins; \u03b2\u2010tubulin III and Map2, on day 15 after cell culture. The real\u2010time polymerase chain reaction (RT\u2010PCR) analysis showed that<jats:italic>p<\/jats:italic>\u2010PCL nanofibrous scaffold could upregulate expression of Map\u20102 and downregulate expression of Nestin genes in nerve cells differentiated from MSCs. This study indicates that mesenchymal stem cell cultured on nanofibrous scaffold have potential differentiation to neuronal cells on and could apply in nerve tissue repair. \u00a9 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1875\u20131881, 2015.<\/jats:p>","DOI":"10.1002\/jbm.a.35328","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T14:09:11Z","timestamp":1410271751000},"page":"1875-1881","source":"Crossref","is-referenced-by-count":64,"title":["Controlled surface morphology and hydrophilicity of<i>polycaprolactone<\/i>toward selective differentiation of mesenchymal stem cells to neural like cells"],"prefix":"10.1002","volume":"103","author":[{"given":"Hoda","family":"Jahani","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Medicine, Ilam University of Medical Sciences Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farid Azizi","family":"Jalilian","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences Faculty of Paramedicine, Ilam University of Medical Sciences Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Yu","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Dentistry, College of Oral Medicine, Taipei Medical University, Division of Oral and Maxillofacial Surgery, Department of Dentistry Taipei Medical University Hospital, Research Center for Biomedical Devices and Prototyping Production Taipei 110 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeid","family":"Kaviani","sequence":"additional","affiliation":[{"name":"Department of Hematology Faculty of Medical Sciences, Tarbiat Modares University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Hematology Faculty of Medical Sciences, Tarbiat Modares University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naghmeh","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng\u2010Liang","family":"Ou","sequence":"additional","affiliation":[{"name":"Nanomedicine Research Center of Taiwan, Research Center for Biomedical Devices and Prototyping Production, Research Center for Biomedical Implants and Microsurgery Devices, Graduate Institute of Biomedical Materials and Engineering, College of Oral Medicine, Taipei Medical University, and Department of Dentistry, Taipei Medical University\u2010Shuang\u2010Ho Hospital Taipei 110 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Hosseinkhani","sequence":"additional","affiliation":[{"name":"Nanomedicine Research Center of Taiwan, Research Center for Biomedical Devices and Prototyping Production, Research Center for Biomedical Implants and Microsurgery Devices, Graduate Institute of Biomedical Materials and Engineering, College of Oral Medicine, Taipei Medical University, and Department of Dentistry, Taipei Medical University\u2010Shuang\u2010Ho Hospital Taipei 110 Taiwan"},{"name":"Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology (Taiwan Tech) Taipei 10607 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4015\/S101623720600018X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.08.062"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2388(200010\/11)19:3<312::AID-SSU13>3.0.CO;2-M"},{"key":"e_1_2_6_5_1","first-page":"1613","volume-title":"Encyclopedia of Biomaterials and Biomedical Engineering","author":"Sanghvi AB","year":"2004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2006.0124"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.01.039"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.05.057"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/19\/45\/455102"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.08.007"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.11.053"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2006.09.006"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.1901"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2009.01.056"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.01.026"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2003.12.004"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0883911506066934"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1093783"},{"key":"e_1_2_6_19_1","first-page":"204","article-title":"Tissue regeneration through self\u2010assembled peptide amphiphile nanofibers","volume":"8","author":"Hosseinkhani H","year":"2006","journal-title":"Yakhteh Med J"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31327"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.08.003"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.03.030"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/107632703322495574"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31304"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.08.017"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr300131h"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.1476"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/cn400075h"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32676"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/107632701753213200"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.435"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30332"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S30434"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1163\/1568562042459733"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.06.051"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.101"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-014-1454-0"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0520"},{"key":"e_1_2_6_39_1","first-page":"587","article-title":"Quantitative method for the analysis of cell attachment using the aligned scaffold structure","volume":"61","author":"Furong T","year":"2007","journal-title":"J Phys"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl048840p"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.03.030"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.05.050"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/1\/1\/002"},{"key":"e_1_2_6_44_1","first-page":"341","article-title":"Design of tissue\u2010engineered nano\u2010scaffold using peptide\u2010amphiphile for regenerative medicine","volume":"2005","author":"Hosseinkhani H","year":"2006","journal-title":"Pept Sci"},{"key":"e_1_2_6_45_1","first-page":"31","article-title":"The effect of aligned and random electrospun fibrous scaffolds on rat mesenchymal stem cell proliferation","volume":"14","author":"Jahani H","year":"2012","journal-title":"Cell J"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.08.062"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.01.002"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2007.00106.x"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-007-3218-z"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/pat.177"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surfcoat.2004.07.062"},{"key":"e_1_2_6_52_1","first-page":"438","article-title":"Enhanced neurite outgrowth by human neurons grown on solid three\u2010dimensional scaffolds","volume":"314","author":"Hayman MW","year":"2004","journal-title":"BBRC"},{"key":"e_1_2_6_53_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.4103\/0022-3859.32215","article-title":"Adult mesenchymal stem cell: Differentiation potential and therapeutic application","volume":"35","author":"Jackson L","year":"2007","journal-title":"J Postgrad Med"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01307"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.08.015"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0149"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7389"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.09.046"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20148"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.2021"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2008.02.009"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4570"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.02.012"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.35328","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.35328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.35328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.a.35328","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.35328","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T10:16:39Z","timestamp":1717323399000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.35328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,24]]},"references-count":62,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1002\/jbm.a.35328"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.35328","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,24]]}}}