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Using phospholipid as a surfactant, BMP\u20102 was modified as a complex (PBC) for dispersing in PLGA\/dichloromethane solution. The PLGA film loaded with BMP\u20102 and phospholipid complex (PBC\u2010PF) showed rough and draped morphology with high entrapment efficiency exceeding 80% and good hydrophilicity, respectively. The<jats:italic>in vitro<\/jats:italic>release study of BMP\u20102 showed that about 50% BMP\u20102 was slowly and continuously released from PBC\u2010PF within 5 weeks and had a short initial burst release only in the last 1.5 days, which was better than serious burst release of PLGA film loaded with pure BMP\u20102 without phospholipid (BMP\u2010PF) as control. By comparison with other PLGA films and tissue culture plates, it was confirmed that PBC\u2010PF significantly promoted the attachment, proliferation, and differentiation of osteoblasts with higher entrapment efficiency and better sustained release. These advantages illustrated that PBC\u2010PF could be a potential substrate providing long\u2010term requisite growth factors for osteoblasts, which might be applied in bone tissue engineering. \u00a9 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2786\u20132796, 2015<\/jats:p>","DOI":"10.1002\/jbm.a.35379","type":"journal-article","created":{"date-parts":[[2015,4,3]],"date-time":"2015-04-03T14:39:48Z","timestamp":1428071988000},"page":"2786-2796","source":"Crossref","is-referenced-by-count":18,"title":["A poly(lactide\u2010co\u2010glycolide) film loaded with abundant bone morphogenetic protein\u20102: A substrate\u2010promoting osteoblast attachment, proliferation, and differentiation in bone tissue engineering"],"prefix":"10.1002","volume":"103","author":[{"given":"Xiangyang","family":"Qu","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory of Child Development and Disorders The Children's Hospital of Chongqing Medical University Chongqing 400014 China"},{"name":"Key Laboratory of Pediatrics in Chongqing Chongqing 400014 China"},{"name":"Chongqing International Science and Technology Cooperation Center for Child Development and Disorders Chongqing 400014 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