{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:31:52Z","timestamp":1761676312381},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National \u201c12th Five-year\u201d High technology Research and Development Program of China","award":["2014AA093605"],"award-info":[{"award-number":["2014AA093605"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In our previous studies, injectable chitosan\u2010based hydrogel (CH) was prepared and its application in surgery removal of tumor was studied. In this study, the antitumor and antimetastasis effects of the macerating solutions from CH were investigated. Our in vitro results showed that macerating solutions from CH significantly increased the proliferation of human normal liver L02 cells. In contrast, macerating solutions from CH showed significant inhibitory effects on the growth of human hepatoma Bel\u20107402 cells. In a mouse H22 tumor model, intraperitoneal injection of macerating solutions from CH decreased tumor growth and prevented tumor diffusion. Tumor weight was decreased dramatically in mice treated with macerating solutions from CH. The thymus index and spleen index were significantly increased by treatment with macerating solutions from CH. Administration of macerating solutions from CH also remarkably increased serum levels of TNF\u2010\u03b1, IL\u20102, IFN\u2010\u03b3, and decreased serum VEGF content as compared with the control group treated with saline. The antimetastasis studies showed that the number of pulmonary nodules, pulmonary metastases index, and lymph nodes index were significantly decreased in experimental groups treated with macerating solutions from CH. This study provided more supporting data for the potential clinical application of CH after surgical removal of tumor. \u00a9 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3879\u20133885, 2015.<\/jats:p>","DOI":"10.1002\/jbm.a.35533","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T23:13:34Z","timestamp":1435619614000},"page":"3879-3885","source":"Crossref","is-referenced-by-count":6,"title":["Antitumor and antimetastasis effects of macerating solutions from an injectable chitosan\u2010based hydrogel on hepatocarcinoma"],"prefix":"10.1002","volume":"103","author":[{"given":"Hui","family":"Wang","sequence":"first","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fulai","family":"Song","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwen","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoqin","family":"Han","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Yushan Road 5 Qingdao 266003 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.08.002"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201033489748"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.yrtph.2009.09.015","article-title":"The safety of chitosan as a pharmaceutical excipient","volume":"56","author":"Baldrick P.","journal-title":"Regul Toxicol Pharmacol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2008.10719671"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.jconrel.2012.03.017","article-title":"Chitosan\u2010based drug nanocarriers: where do we stand?","volume":"161","author":"Garcia\u2010Fuentes M","journal-title":"J Control Release"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10047-005-0313-0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2004.11.009"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.4.945"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-8130(02)00064-8"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2005.02.028"},{"key":"e_1_2_5_12_1","unstructured":"SongFL ShaoK LiuWS HanBQ MaHN.valuation of a chitosan\u2010based in situ forming hydrogel.J Funct Mat2014(E):09065\u201309069"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.colsurfb.2010.07.043","article-title":"An in situ formed biodegradable hydrogel for reconstruction of the corneal endothelium","volume":"82","author":"Liang Y","journal-title":"Colloids Surf B Biointerfaces"},{"key":"e_1_2_5_14_1","first-page":"2809","article-title":"Sensitivity of K562 and HL\u201060 cells to edelfosine, an ether lipid drug, correlates with production of reactive oxygen species","volume":"58","author":"Wagner BA","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e31822b02f4"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2006.06.003"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0939-6411(03)00095-X"},{"key":"e_1_2_5_18_1","first-page":"78","article-title":"Anti\u2010tumor effects of Astragalus on hepatocellular carcinoma in vivo","volume":"44","author":"Li LK","journal-title":"Indian J Pharmacol"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00465-9"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.01.020"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.05.031"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1994.tb02904.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2007.11.018"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.03.022"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.35533","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.35533","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T23:57:40Z","timestamp":1696377460000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.35533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,14]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1002\/jbm.a.35533"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.35533","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,14]]}}}