{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:07:14Z","timestamp":1791821234873,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T00:00:00Z","timestamp":1450915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11172338"],"award-info":[{"award-number":["11172338"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To promote bone healing, bone repair biomaterials are increasingly designed to incorporate growth factors. However, the impact of matrix mechanics of cell\u2010free scaffold independent of microstructure on the osteogenic differentiation of endogenous osteoprogenitor cells orchestrating bone repair and regeneration remains not to be fully understood. In our recent study, three\u2010dimensional (3D) scaffolds with different stiffness but same microstructure have been successfully fabricated by coating decellularized bone with collagen\/hydroxyapatite (HA) mixture with different collagen rations. It has been demonstrated that the scaffold with optimal stiffness can induce the osteogenic differentiation of MSCs <jats:italic>in vitro<\/jats:italic> and in the subcutaneous tissue. The present <jats:italic>in vivo<\/jats:italic> study further investigated the repair efficiency of these scaffolds in a rabbit radius with a critical\u2010sized segmental defect model and its potential mechanism. Micro\u2010computed tomography (\u03bc\u2010CT), X\u2010ray and histological analysis were carried out to evaluate the repair capacity of these scaffolds. The results demonstrated that the cell\u2010free scaffold with optimal stiffness incorporation of endogenous osteoprogenitor cells significantly promoted the repair and reconstruction quality of mass bone defect. One of the crucial mechanisms was that hypoxia and stromal cell\u2010derived factor\u20101\u03b1 (SDF\u20101\u03b1) mediated mesenchymal stem cells (MSCs) migration by which matrix mechanics exerted influence on bone fracture healing. These findings suggested that only modulating the matrix stiffness of cell\u2010free scaffold can be one of the most attractive strategies for promoting the progression of bone healing. \u00a9 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 833\u2013841, 2016.<\/jats:p>","DOI":"10.1002\/jbm.a.35622","type":"journal-article","created":{"date-parts":[[2015,12,10]],"date-time":"2015-12-10T01:57:46Z","timestamp":1449712666000},"page":"833-841","source":"Crossref","is-referenced-by-count":36,"title":["Cell\u2010free scaffolds with different stiffness but same microstructure promote bone regeneration in rabbit large bone defect model"],"prefix":"10.1002","volume":"104","author":[{"given":"Guobao","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University Chongqing 400044 People's Republic of China"},{"name":"Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University Chongqing 400044 People's Republic of 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