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Here, the obtained titanium alloy implant (TC4\/LBL\/CT\/BMP2) can regulate the release of loaded calcitonin and BMP2 agents in a sustaining manner to accelerate the bone formation and simultaneously inhibit bone resorption. <jats:italic>In vitro<\/jats:italic> results show that the bone\u2010related cells on TC4\/LBL\/CT\/BMP2 present the lowest production level of tartrate resistant acid phosphatase (TRAP) but the highest (<jats:italic>p &lt;<\/jats:italic> 0.05) level of alkaline phosphatase (ALP) activity, osteocalcin production, mineralization capacity and osteoblast\u2010related gene expression among all groups after treatment for 7 or 21 days, respectively. Besides, <jats:italic>in vivo<\/jats:italic> studies of micro\u2010CT analysis, routine histological and immunohistochemical analysis also collectively demonstrate that the TC4\/LBL\/CT\/BMP2 implant can dramatically promote the formation and remodeling of new bone in osteoporotic rabbits after implantation for 30 days and 90 days, respectively. <jats:italic>In vivo<\/jats:italic> push\u2010out testing further confirms that the TC4\/LBL\/CT\/BMP2 implant has the highest (<jats:italic>p &lt;<\/jats:italic> 0.01) interfacial shear strength and favorable bone\u2010implant osseointegration. Overall, this study establishes a simple and profound methodology to fabricate a biofunctional TC4 implant for the treatment of local osteoporotic fractures <jats:italic>in vivo<\/jats:italic>. \u00a9 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1437\u20131451, 2016.<\/jats:p>","DOI":"10.1002\/jbm.a.35667","type":"journal-article","created":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T03:35:53Z","timestamp":1454038553000},"page":"1437-1451","source":"Crossref","is-referenced-by-count":38,"title":["Enhancement of local bone remodeling in osteoporotic rabbits by biomimic multilayered structures on <scp>T<\/scp>i6<scp>A<\/scp>l4<scp>V<\/scp> implants"],"prefix":"10.1002","volume":"104","author":[{"given":"Ling","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Life Science Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinkun","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghuan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Xinqiao Hospital, Third Military Medical University Xinqiao Street Chongqing 400037 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Xinqiao Hospital, Third Military Medical University Xinqiao Street Chongqing 400037 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyong","family":"Cai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biorheological Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing 400044 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.03.037"},{"key":"e_1_2_6_3_1","first-page":"3","article-title":"The relationship between the mechanisms of tooth and implant support","volume":"5","author":"Picton DC","year":"1974","journal-title":"Oral Sci Rev"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.71"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2015.02.018"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.02.033"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","unstructured":"AlghamdiHS vanden BeuckenJJJP JansenJA.Osteoporosis fracture healing and osseointegration. 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