{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:00:55Z","timestamp":1790971255186,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,9]],"date-time":"2016-04-09T00:00:00Z","timestamp":1460160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Stem cell\u2010based therapies hold great promise for enhancing tissue regeneration. However, the majority of cells die shortly after transplantation, which greatly diminishes the efficacy of stem cell\u2010based therapies. Poor cell engraftment and survival remain a major bottleneck to fully exploiting the power of stem cells for regenerative medicine. Biomaterials such as hydrogels can serve as artificial matrices to protect cells during delivery and guide desirable cell fates. However, conventional hydrogels often lack macroporosity, which restricts cell proliferation and delays matrix deposition. Here we report the use of injectable, macroporous microribbon (\u03bcRB) hydrogels as stem cell carriers for bone repair, which supports direct cell encapsulation into a macroporous scaffold with rapid spreading. When transplanted in a critical\u2010sized, mouse cranial defect model, \u03bcRB\u2010based hydrogels significantly enhanced the survival of transplanted adipose\u2010derived stromal cells (ADSCs) (81%) and enabled up to three\u2010fold cell proliferation after 7 days. In contrast, conventional hydrogels only led to 27% cell survival, which continued to decrease over time. MicroCT imaging showed \u03bcRBs enhanced and accelerated mineralized bone repair compared to hydrogels (61% vs. 34% by week 6), and stem cells were required for bone repair to occur. These results suggest that paracrine signaling of transplanted stem cells are responsible for the observed bone repair, and enhancing cell survival and proliferation using \u03bcRBs further promoted the paracrine\u2010signaling effects of ADSCs for stimulating endogenous bone repair. We envision \u03bcRB\u2010based scaffolds can be broadly useful as a novel scaffold for enhancing stem cell survival and regeneration of other tissue types. \u00a9 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1321\u20131331, 2016.<\/jats:p>","DOI":"10.1002\/jbm.a.35715","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T02:59:45Z","timestamp":1458356385000},"page":"1321-1331","source":"Crossref","is-referenced-by-count":40,"title":["Winner of the Young Investigator Award of the Society for Biomaterials at the 10th World Biomaterials Congress, May 17\u201322, 2016, Montreal QC, Canada: Microribbon\u2010based hydrogels accelerate stem cell\u2010based bone regeneration in a mouse critical\u2010size cranial defect model"],"prefix":"10.1002","volume":"104","author":[{"given":"Li\u2010Hsin","family":"Han","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University School of Medicine 300 Pasteur Dr, Edward Building Room 114 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan","family":"Conrad","sequence":"additional","affiliation":[{"name":"Program of Stem Cell Biology and Regenerative Medicine, Stanford University 300 Pasteur Dr, Edward Building Room 114 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery Stanford University School of Medicine 257 Campus Dr, Hagey Building Room GK106 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Deveza","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University School of Medicine 300 Pasteur Dr, Edward Building Room 114 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University School of Medicine 300 Pasteur Dr, Edward Building Room 114 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Stanford University School of Medicine 300 Pasteur Dr, Grant Building Room H307A Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manish J.","family":"Butte","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Stanford University School of Medicine 300 Pasteur Dr, Grant Building Room H307A Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Longaker","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine 257 Campus Dr, Hagey Building Room GK106 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derrick","family":"Wan","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery Stanford University School of Medicine 257 Campus Dr, Hagey Building Room GK106 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University School of Medicine 300 Pasteur Dr, Edward Building Room 114 Stanford California 94305"},{"name":"Department of Bioengineering Stanford University 300 Pasteur Dr, Edward Building Room 114 Stanford California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surge.2012.04.001"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3928\/0147-7447-20020502-04"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0589"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-1100fje"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1201830109"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218052109"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321717111"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01230.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0714"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24497"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cm101391y"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.11.002"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1421"},{"key":"e_1_2_5_15_1","first-page":"161","volume-title":"Skeletal Tissue Regeneration. 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