{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T06:32:17Z","timestamp":1778394737570,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,6,14]],"date-time":"2016-06-14T00:00:00Z","timestamp":1465862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polyethylene micro\u2010sized wear particles released from orthopedic implants promote inflammation and osteolysis; however, less is known about the bioactivity of polyethylene nanosized wear particles released from the last generation of polymer\u2010bearing surfaces. We aim to assess the internalization of fluorescent polyethylene\u2010like nanoparticles by both human macrophages and osteoclasts and also, to determine their effects in osteoclastogenesis <jats:italic>in vitro<\/jats:italic>. Human macrophages and osteoclasts were incubated with several ratios of fluorescent polyethylene\u2010like nanoparticles between 1 and 72 h, and 4 h, 2, 4, 6, and 9 days, respectively. The internalization of nanoparticles was quantified by flow cytometry and followed by both confocal and video time\u2010lapse microscopy. Osteoclast differentiation and activity was semiquantified by tartrate\u2010resistant acid phosphatase (TRAP) staining, TRAP mRNA relative expression, and pit resorption assay, respectively. Macrophages, osteoclast precursors and mature osteoclasts internalized nanoparticles in a dose\u2010 and time\u2010dependent manner and maintained their resorptive activity. In addition, nanoparticles significantly increased the osteoclastogenesis as shown by upregulation of the TRAP expressing cell number. We conclude that polyethylene\u2010like nanosized wear particles promote osteoclast differentiation without alteration of bone resorptive activity of mature osteoclasts and they could be considered as important actors in periprosthetic osteolysis of the last new generation of polymer\u2010bearing surfaces. \u00a9 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2649\u20132657, 2016.<\/jats:p>","DOI":"10.1002\/jbm.a.35803","type":"journal-article","created":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T17:07:44Z","timestamp":1464887264000},"page":"2649-2657","source":"Crossref","is-referenced-by-count":11,"title":["Pro\u2010osteoclastic <i>in vitro<\/i> effect of Polyethylene\u2010like nanoparticles: Involvement in the pathogenesis of implant aseptic loosening"],"prefix":"10.1002","volume":"104","author":[{"given":"Kevin","family":"Brulefert","sequence":"first","affiliation":[{"name":"Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours INSERM, UMR 957 Nantes 44035 France"},{"name":"University of Nantes, Nantes Atlantique Universities Nantes France"},{"name":"Nantes University Hospital Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"C\u00f3rdova","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours INSERM, UMR 957 Nantes 44035 France"},{"name":"University of Nantes, Nantes Atlantique Universities Nantes France"},{"name":"Department of Oral and Maxillofacial Surgery\u2014Faculty of Dentistry University of Chile\u2014Conicyt Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e9n\u00e9dicte","family":"Brulin","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours INSERM, UMR 957 Nantes 44035 France"},{"name":"University of Nantes, Nantes Atlantique Universities Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"Faucon","sequence":"additional","affiliation":[{"name":"CEISAM\u2013UMR CNRS 6230 University of Nantes Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipe","family":"Hulin","sequence":"additional","affiliation":[{"name":"Nantes University Hospital Nantes France"},{"name":"MicroPICell Platform, SFR Sant\u00e9 Fran\u00e7ois Bonamy INSERM, UMS 016\u2010UMS CNRS 3556 Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Nedellec","sequence":"additional","affiliation":[{"name":"Nantes University Hospital Nantes France"},{"name":"MicroPICell Platform, SFR Sant\u00e9 Fran\u00e7ois Bonamy INSERM, UMS 016\u2010UMS CNRS 3556 Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Gouin","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours INSERM, UMR 957 Nantes 44035 France"},{"name":"University of Nantes, Nantes Atlantique Universities Nantes France"},{"name":"Nantes University Hospital Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"Passuti","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours INSERM, UMR 957 Nantes 44035 France"},{"name":"University of Nantes, Nantes Atlantique Universities Nantes France"},{"name":"Nantes University Hospital Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El\u00e9na","family":"Ishow","sequence":"additional","affiliation":[{"name":"CEISAM\u2013UMR CNRS 6230 University of Nantes Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Heymann","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours INSERM, UMR 957 Nantes 44035 France"},{"name":"University of Nantes, Nantes Atlantique Universities Nantes France"},{"name":"Nantes University Hospital Nantes France"},{"name":"Department of Oncology and Metabolism University of Sheffield, The Medical School Sheffield England United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2011.128"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000238813.95035.1b"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.10.042"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-014-3735-2"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wear.2005.02.035"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2013.7525"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.09.023"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.04.011"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.90B8.20737"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cp51369f"},{"key":"e_1_2_5_12_1","first-page":"3:13268","article-title":"Solvatochromic dissociation of non\u2010covalent fluorescent organic nanoparticles upon cell internalization","author":"Breton M","year":"2011","journal-title":"Chem Mater"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4TB01199F"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1719"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2684"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2170"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1007\/s00018-003-3020-0","article-title":"Regulation of macrophage activation","volume":"60","author":"Ma J","year":"2003","journal-title":"Cell Mol Life Sci CMLS"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(200009)53:5<490::AID-JBM7>3.0.CO;2-7"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(200011)52:2<296::AID-JBM8>3.0.CO;2-9"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbbm.2006.05.011"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.054"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200800123"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801763105"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/macp.200500372"},{"key":"e_1_2_5_26_1","first-page":"871","article-title":"Cellular mechanisms of calcium phosphate ceramic degradation","volume":"14","author":"Heymann D","year":"1999","journal-title":"Histol Histopathol"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/44.10.8813078"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1631"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1754"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/214878"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20714"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.223669"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-010-1645-5"},{"key":"e_1_2_5_34_1","first-page":"277","article-title":"Orthopaedic implant failure: aseptic implant loosening\u2010the contribution and future challenges of mouse models in translational research","volume":"127","author":"Cordova LA","year":"2014","journal-title":"Clin Sci Lond Engl 1979"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-013-1887-4"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.35803","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.35803","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.35803","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T21:52:34Z","timestamp":1694296354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.35803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,14]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/jbm.a.35803"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.35803","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,14]]}}}