{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:31:21Z","timestamp":1788543081204,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["K2015\u201052X\u20100949528\u20104"],"award-info":[{"award-number":["K2015\u201052X\u20100949528\u20104"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003744","name":"Stiftelsen Handlanden Hjalmar Svenssons","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003744","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The breach of the skin barrier is a critical issue associated with the treatment of individuals with transfemoral amputation (TFA) using osseointegrated, percutaneous titanium implants. Thirty TFA patients scheduled for abutment exchange or removal were consecutively enrolled. The aims were to determine the macroscopic skin signs, the presence of bacteria and the gene expression in abutment\u2010adherent cells and to conduct correlative and comparative analyses between the different parameters. Redness and a granulation ring were present in 47% of the patients. Bacteria were detected in 27\/30 patients, commonly in the bone canal. <jats:italic>Staphylococcus aureus<\/jats:italic>, coagulase\u2010negative staphylococci, streptococci, and <jats:italic>Enterococcus faecalis<\/jats:italic> were the most common. A positive correlation was found between TNF\u2010\u03b1 expression and the detection of <jats:italic>S. aureus<\/jats:italic>. <jats:italic>Staphylococcus aureus<\/jats:italic> together with other bacterial species revealed a positive relationship with MMP\u20108 expression. A negative correlation was demonstrated between the length of the residual femur bone and the detection of a granulation ring and <jats:italic>E. faecalis<\/jats:italic>. A positive correlation was revealed between fixture loosening and pain and the radiological detection of endosteal bone resorption. Fixture loosening was also correlated with the reduced expression of interleukin\u201010 and osteocalcin. It is concluded that several relationships exist between clinical, radiological, microbiological, and molecular assessments of the percutaneous area of TFAs. Further long term studies on larger patient cohorts are required to determine the precise cause\u2010effect relationships and unravel the role of host\u2010bacteria interactions in the skin, bone canal and on the abutment for the longevity of percutaneous implants as treatment of TFA. \u00a9 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 578\u2013589, 2017.<\/jats:p>","DOI":"10.1002\/jbm.a.35935","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T22:47:38Z","timestamp":1476744458000},"page":"578-589","source":"Crossref","is-referenced-by-count":40,"title":["The clinical, radiological, microbiological, and molecular profile of the skin\u2010penetration site of transfemoral amputees treated with bone\u2010anchored prostheses"],"prefix":"10.1002","volume":"105","author":[{"given":"Maria","family":"Lenner\u00e5s","sequence":"first","affiliation":[{"name":"Department of Biomaterials Institute of Clinical Sciences Sahlgrenska Academy University of Gothenburg Gothenburg SE\u2010405 30 Sweden Box 412"},{"name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy Gothenburg SE\u2010405 30, Sweden Box 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Sahlgrenska Academy University of Gothenburg Gothenburg SE\u2010405 30 Sweden Box 412"},{"name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy Gothenburg SE\u2010405 30, Sweden Box 412"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Forugh","family":"Vazirisani","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Institute of Clinical Sciences Sahlgrenska Academy University of Gothenburg Gothenburg SE\u2010405 30 Sweden Box 412"},{"name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy Gothenburg SE\u2010405 30, Sweden Box 412"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Palmquist","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Institute of Clinical Sciences Sahlgrenska Academy University of Gothenburg Gothenburg SE\u2010405 30 Sweden Box 412"},{"name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy Gothenburg SE\u2010405 30, Sweden Box 412"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6rjan","family":"Berlin","sequence":"additional","affiliation":[{"name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy Gothenburg SE\u2010405 30, Sweden Box 412"},{"name":"Department of Orthopaedics Institute of Clinical Sciences Sahlgrenska Academy University of Gothenburg Gothenburg Sweden"},{"name":"Centre for Advanced Reconstruction of Extremities (CARE) Sahlgrenska University Hospital M\u00f6lndal, SE\u2010431 30 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rickard","family":"Br\u00e5nemark","sequence":"additional","affiliation":[{"name":"BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy Gothenburg SE\u2010405 30, Sweden Box 412"},{"name":"Department of Orthopaedics Institute of Clinical Sciences Sahlgrenska Academy University of Gothenburg Gothenburg Sweden"},{"name":"Centre for Advanced Reconstruction of Extremities (CARE) 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