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Here, we used this model to determine the role of angiogenesis in post\u2010suturectomy resynostosis and examine whether controlled release of angiogenesis inhibitors could delay bone regeneration. Hydrogels [DB\u2010co\u2010PEG\/poly (TEGDMA)\u2010co\u2010(N3\u2010TEGDMA)] were produced containing anti\u2010angiogenic compounds [anti\u2010VEGFA\u2010antibody or hypoxia inducible factor 1\u03b1\u2010inhibitor topotecan]. Bioactivity <jats:italic>in vitro<\/jats:italic> was assessed by tube length and branching points of endothelial cells in hydrogel\u2010conditioned media. <jats:italic>In vivo<\/jats:italic> effects were examined 14 day post\u2010suturectomy, based on the temporal analysis of angiogenic mRNAs during resynostosis following posterior frontal suture removal. MicroCT was used to quantify angiogenesis in contrast\u2010agent\u2010perfused blood vessels and bone defect size in defects receiving hydrogel, anti\u2010VEGFA\/hydrogel, or topotecan\/hydrogel. Shorter endothelial tube length and less branching were seen in inhibitor\u2010conditioned media (topotecan\u2009&gt;\u2009AbVEGFA). <jats:italic>In vivo<\/jats:italic>, both compounds inhibited angiogenesis compared with hydrogel\u2010only. Anti\u2010VEGFA\/hydrogel reduced resynostosis compared with empty defects, but topotecan\/hydrogel blocked bone regeneration. We demonstrate that anti\u2010angiogenic compounds can be incorporated into a spontaneously polymerizing hydrogel and remain active over 14 days <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. Moreover, bone formation can be delayed by inhibiting neovascularization, suggesting possible use as a therapeutic to control resynostosis following suturectomies and potential applications in other conditions where rapid osteogenesis is not desired. \u00a9 2017 Wiley Periodicals Inc. 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