{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:37:56Z","timestamp":1761129476942},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T00:00:00Z","timestamp":1507680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mastoidectomy, the removal of infected mastoid bones, is a common surgical procedure for the treatment of chronic otitis media. Persistent and recurrent otorrhea and accumulation of keratin debris following open cavity mastoidectomy are still bothersome issues for both patients and otologists. In this study, we used human ear adipose\u2010derived stromal cells (hEASCs) in combination with polycaprolactone (PCL) scaffolds and osteogenic differentiation medium (ODM) to regenerate temporal bone defects. The hEASCs showed stem cell phenotypes, and these characteristics were maintained up to passage 5. Mastoid bulla and cranial bone defects were induced in Sprague\u2010Dawley rats using AgNO<jats:sub>3<\/jats:sub> and burr hole drilling, respectively, and the rats were then divided into five groups: (1) control, (2) hEASCs, (3) hEASCs\u2009+\u2009ODM, (4) hEASCs\u2009+\u2009PCL scaffolds, and (5) hEASCs\u2009+\u2009PCL scaffolds\u2009+\u2009ODM. Osteogenesis was evaluated by micro\u2010computed tomography and histology. Compared with the control group, the groups transplanted with hEASCs and PCL scaffolds had significantly higher bone formation along the periphery of the mastoid bulla area. Moreover, ODM synergistically enhanced bone formation in mastoid bulla defects. Our results suggest that combining hEASCs with PCL scaffolds represents a promising method for anatomical and functional reconstruction of postoperative temporal bone defects following mastoidectomy. \u00a9 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3493\u20133501, 2017.<\/jats:p>","DOI":"10.1002\/jbm.a.36194","type":"journal-article","created":{"date-parts":[[2017,9,6]],"date-time":"2017-09-06T01:17:15Z","timestamp":1504660635000},"page":"3493-3501","source":"Crossref","is-referenced-by-count":9,"title":["Osteogenesis for postoperative temporal bone defects using human ear adipose\u2010derived stromal cells and tissue engineering: An animal model study"],"prefix":"10.1002","volume":"105","author":[{"given":"Yeon Ju","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Ajou University School of Medicine, San 5 Woncheon\u2010dong, Yeongtong\u2010gu Suwon 443\u2010721 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Gu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Ajou University School of Medicine, San 5 Woncheon\u2010dong, Yeongtong\u2010gu Suwon 443\u2010721 Republic of 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Jilin 133000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oak\u2010Sung","family":"Choo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Ajou University School of Medicine, San 5 Woncheon\u2010dong, Yeongtong\u2010gu Suwon 443\u2010721 Republic of Korea"},{"name":"Department of Medical Sciences Ajou University Graduate School of Medicine, San 5 Woncheon\u2010dong Yeongtong\u2010gu Suwon 443\u2010721 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang Yun","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Ajou University School of Medicine, San 5 Woncheon\u2010dong Yeongtong\u2010gu Suwon 443\u2010721 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byoung Hyun","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Ajou University School of Medicine, San 5 Woncheon\u2010dong 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