{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:14:35Z","timestamp":1787163275999,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Maryland Stem Cell Research Fund and Hartwell Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tissue extracellular matrix (ECM) is a complex material made up of fibrous proteins and ground substance (glycosaminoglycans, GAGs) that are secreted by cells. ECM contains important biological cues that modulate cell behaviors, and it also serves as a structural scaffold to which cells can adhere. For clinical applications, where immune rejection is a constraint, ECM can be processed using decellularization methods intended to remove cells and donor antigens from tissue or organs, while preserving native biological cues essential for cell growth and differentiation. In this study, a decellularized ECM\u2010based composite hydrogel was formulated by using modified GAGs that covalently bind tissue particles. These GAG\u2013ECM composite hydrogels combine the advantages of solid decellularized ECM scaffolds and pepsin\u2010digested ECM hydrogels by facilitating ECM hydrogel formation without a disruptive enzymatic digestion process. Additionally, engineered hydrogels can contain more than one type of ECM (from bone, fat, liver, lung, spleen, cartilage, or brain), at various concentrations. These hydrogels demonstrated tunable gelation kinetics and mechanical properties, offering the possibility of numerous\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    applications with different property requirements. Retained bioactivity of ECM particles crosslinked into this hydrogel platform was confirmed by the variable response of stem cells to different types of ECM particles with respect to osteogenic differentiation\n                    <jats:italic>in vitro<\/jats:italic>\n                    , and bone regeneration\n                    <jats:italic>in vivo<\/jats:italic>\n                    . \u00a9 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 147\u2013159, 2018.\n                  <\/jats:p>","DOI":"10.1002\/jbm.a.36218","type":"journal-article","created":{"date-parts":[[2017,9,6]],"date-time":"2017-09-06T21:24:54Z","timestamp":1504733094000},"page":"147-159","source":"Crossref","is-referenced-by-count":66,"title":["Extracellular matrix particle\u2013glycosaminoglycan composite hydrogels for regenerative medicine applications"],"prefix":"10.1002","volume":"106","author":[{"given":"Vince","family":"Beachley","sequence":"first","affiliation":[{"name":"Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering Johns Hopkins University Baltimore Maryland 21287"},{"name":"Department of Biomedical Engineering Rowan University Glassboro New Jersey 08028"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garret","family":"Ma","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering Johns Hopkins University Baltimore Maryland 21287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Papadimitriou","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering Johns Hopkins University Baltimore Maryland 21287"},{"name":"German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Arnold Str. 18 Dresden 01307 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Gibson","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering Johns Hopkins University Baltimore Maryland 21287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Corvelli","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering Johns Hopkins University Baltimore Maryland 21287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Elisseeff","sequence":"additional","affiliation":[{"name":"Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering Johns Hopkins University Baltimore Maryland 21287"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.3.205s"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3620"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2328"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-071910-124743"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0437"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1684"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2170"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189345"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3154"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3619"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008085"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61598-6"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.11.101"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.09.013"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.04.042"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.051"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.10.062"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.51.6.787"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90012-8"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.07.058"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.12.033"},{"key":"e_1_2_7_24_1","first-page":"3675","article-title":"Decellularization of tissues and organs","volume":"27","author":"Gilbert TW","year":"2006","journal-title":"Biomaterials"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e31824ec3dc"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/469120"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0578"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.355"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35143"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(97)00225-8"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.media.2010.06.010"},{"key":"e_1_2_7_32_1","first-page":"1394","article-title":"Assessment of portal hypertension and high\u2010risk oesophageal varices with liver and spleen three\u2010dimensional multifrequency MR elastography in liver cirrhosis","volume":"24","author":"Ronot M","year":"2014","journal-title":"Eur Radiol"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1497"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.89.1.3"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.01.040"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004097107"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.04.029"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.02.032"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0sm00642d"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.36218","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T10:25:18Z","timestamp":1695983118000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.36218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,26]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1002\/jbm.a.36218"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.36218","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,26]]}}}