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Human ADSCs were transduced with a LV vector carrying the cDNA for BMP\u20102. The transduced cells were loaded onto a 3D printed Hyperelastic \u201cBone\u201d (HB) scaffold.<jats:italic>In vitro<\/jats:italic>BMP\u20102 production was assessed using enzyme\u2010linked immunosorbent assay analysis. The ability of ADSCs loaded on the HB scaffold to induce<jats:italic>in vivo<\/jats:italic>bone formation in a hind limb muscle pouch model was assessed in the following groups: ADSCs transduced with LV\u2010BMP\u20102, LV\u2010green fluorescent protein, ADSCs alone, and empty HB scaffolds. Bone formation was assessed using radiographs, histology and histomorphometry. Transduced ADSCs BMP\u20102 production on the HB scaffold at 24 hours was similar on 3D printed HB scaffolds versus control wells with transduced cells alone, and continued to increase after 1 and 2 weeks of culture. Bone formation was noted in LV\u2010BMP\u20102 animals on plain radiographs at 2 and 4 weeks after implantation; no bone formation was noted in the other groups. Histology demonstrated that the LV\u2010BMP\u20102 group was the only group that formed woven bone and the mean bone area\/tissue area was significantly greater when compared with the other groups. 3D printed HB scaffolds are effective carriers for transduced ADSCs to promote bone repair. The combination of gene therapy and tissue engineered scaffolds is a promising multidisciplinary approach to bone repair with significant clinical potential. \u00a9 2018 Wiley Periodicals, Inc. 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