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The successful conjugating of MTX onto the nanoparticles (NPs) was confirmed by X\u2010ray diffraction, thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, vibrating sample magnetometer, and transmission electron microscopy techniques. The results showed that the average size was 46.82\u2009\u00b1\u20095.03 nm. This target drug delivery system is dependent on the release of the MTX within the lysosomal compartment. Hemolysis assay and cytotoxicity study results on HFF\u20102 and HEK\u2010293 cell lines show that as prepared MNPs are biocompatible. The cytotoxicity of void of the MTX and F\u2010Gly\u2010MTX NPs were compared to each other by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide assay of the treated MCF\u20107 cell line. Enzymatic release studies exhibited the release of the MTX via peptide bond cleavage in the presence of proteinase K. These studies specify that the F\u2010Gly\u2010MTX NPs have a very remarkable anticancer effect, for breast cancer cell line. \u00a9 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1646\u20131654, 2018.<\/jats:p>","DOI":"10.1002\/jbm.a.36364","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T08:26:37Z","timestamp":1518596797000},"page":"1646-1654","source":"Crossref","is-referenced-by-count":70,"title":["Enzymatic stimuli\u2010responsive methotrexate\u2010conjugated magnetic nanoparticles for target delivery to breast cancer cells and release study in lysosomal condition"],"prefix":"10.1002","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7487-8188","authenticated-orcid":false,"given":"Hamed","family":"Nosrati","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biomaterials, School of Pharmacy Zanjan University of Medical Sciences Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Mojtahedi","sequence":"additional","affiliation":[{"name":"Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of 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