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In particular, Interferon\u2010\u03b3 (IFN\u2010\u03b3) is a critical endogenous mediator of inflammation and plays a key role in a wide variety of biological responses including tissue healing. However, the contribution of endogenous IFN\u2010\u03b3 on different features of the foreign body response induced by synthetic implants regarding neovascularization, inflammation, and fibrogenesis is not well known. Here, we evaluated inflammatory angiogenesis and fibrogenesis induced by implantation of polyether\u2013polyurethane sponges in mice targeted disrupted of the interferon\u2010\u03b3 gene (IFN\u2010\u03b3<jats:sup>\u2212\/\u2212<\/jats:sup>) and wild\u2010type (WT). The hemoglobin content, the number of vessels, and blood flow (evaluated by LDPI\u2014laser Doppler perfusion imaging) were decreased in the implants from IFN\u2010\u03b3<jats:sup>\u2212\/\u2212<\/jats:sup> as compared to WT mice. Likewise, neutrophils and macrophages accumulation (MPO and NAG activities, respectively) was decreased in IFN\u2010\u03b3<jats:sup>\u2212\/\u2212<\/jats:sup> implants. Interestingly, while the local content of VEGF, TNF\u2010\u03b1, CXCL\u20101\/KC, as measured by ELISA, and iNOS expression, as measured by qPCR, were significantly reduced, the content of IL\u201010 was greatly increased in the implants from IFN\u2010\u03b3<jats:sup>\u2212\/\u2212<\/jats:sup> mice as compared to WT mice. No alterations were observed in CCL\u20102\/MCP\u20101 levels. Lastly, the collagen deposition, assessed by Picro\u2010Sirius red\u2010stained histological sections, was also reduced in IFN\u2010\u03b3<jats:sup>\u2212\/\u2212<\/jats:sup> implants. Altogether, these data suggest that IFN\u2010\u03b3 activity contributes to inflammatory angiogenesis and fibrogenesis in synthetic implants and that lack of IFN\u2010\u03b3 expression attenuates foreign body reaction to implants in mice. \u00a9 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2243\u20102250, 2018.<\/jats:p>","DOI":"10.1002\/jbm.a.36416","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T01:34:20Z","timestamp":1522028060000},"page":"2243-2250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Lack of interferon\u2010gamma attenuates foreign body reaction to subcutaneous implants in mice"],"prefix":"10.1002","volume":"106","author":[{"given":"Puebla","family":"Cassini\u2010Vieira","sequence":"first","affiliation":[{"name":"Departamentos de Fisiologia e Biof\u00edsica Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais 31270\u2010901 Belo Horizonte Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"de Carvalho Santuchi","sequence":"additional","affiliation":[{"name":"Departamentos de Fisiologia e Biof\u00edsica Instituto de Ci\u00eancias Biol\u00f3gicas, 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