{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T12:28:32Z","timestamp":1782563312276,"version":"3.54.5"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DMR 0239744"],"award-info":[{"award-number":["DMR 0239744"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002369","name":"University of Delaware Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002369","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["P20\u2010RR017716"],"award-info":[{"award-number":["P20\u2010RR017716"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["P20\u2010RR015588"],"award-info":[{"award-number":["P20\u2010RR015588"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["RO1 DC4336"],"award-info":[{"award-number":["RO1 DC4336"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["RO1 DC011377"],"award-info":[{"award-number":["RO1 DC011377"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vocal folds are connective tissues housed in the larynx, which can be subjected to various injuries and traumatic stimuli that lead to aberrant tissue structural alterations and fibrotic\u2010induced biomechanical stiffening observed in patients with voice disorders. Much effort has been devoted to generate soft biomaterials that are injectable directly to sites of injury. To date, materials applied toward these applications have been largely focused on natural extracellular matrix\u2010derived materials such as collagen, fibrin or hyaluronic acid; these approaches have suffered from the fact that materials are not sufficiently robust mechanically nor offer sufficient flexibility to modulate material properties for targeted injection. We have recently developed multiple resilin\u2010inspired elastomeric hydrogels that possess similar mechanical properties as those reported for vocal fold tissues, and that also show promising <jats:italic>in vitro<\/jats:italic> cytocompatibility and <jats:italic>in vivo<\/jats:italic> biocompatibility. Here we report studies that test the delivery of resilin\u2010based hydrogels through injection to the subcutaneous tissue in a wild\u2010type mice model; histological and genetic expression outcomes were monitored. The rapid kinetics of crosslinking enabled facile injection and ensured the rapid transition of the viscous resilin precursor solution to a solid\u2010like hydrogel in the subcutaneous space <jats:italic>in vivo<\/jats:italic>; the materials exhibited storage shear moduli in the range of 1000\u20132000 Pa when characterized through oscillatory rheology. Histological staining and gene expression profiles suggested minimal inflammatory profiles three weeks after injection, thereby demonstrating the potential suitability for site\u2010specific <jats:italic>in vivo<\/jats:italic> injection of these elastomeric materials. \u00a9 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2229\u20102242, 2018.<\/jats:p>","DOI":"10.1002\/jbm.a.36418","type":"journal-article","created":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T14:37:59Z","timestamp":1522766279000},"page":"2229-2242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Biocompatibility of injectable resilin\u2010based hydrogels"],"prefix":"10.1002","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3003-9902","authenticated-orcid":false,"given":"Linqing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering University of Delaware Newark Delaware 19716"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanna M.","family":"Stiadle","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology\u2010Head and Neck Surgery, Department of Surgery University of Wisconsin\u2010Madison, 5136 WIMR, 1111 Highland Ave Madison Wisconsin 53792"},{"name":"Department of Communication Sciences and Disorders University of Wisconsin\u2010Madison Madison Wisconsin 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth E.","family":"Levendoski","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology\u2010Head and Neck Surgery, Department of Surgery University of Wisconsin\u2010Madison, 5136 WIMR, 1111 Highland Ave Madison Wisconsin 53792"},{"name":"Department of Communication Sciences and Disorders University of Wisconsin\u2010Madison Madison Wisconsin 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang K.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering University of Delaware Newark Delaware 19716"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Thibeault","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology\u2010Head and Neck Surgery, Department of Surgery University of Wisconsin\u2010Madison, 5136 WIMR, 1111 Highland Ave Madison Wisconsin 53792"},{"name":"Department of Communication Sciences and Disorders University of Wisconsin\u2010Madison Madison Wisconsin 53792"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristi L.","family":"Kiick","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering University of Delaware Newark Delaware 19716"},{"name":"Department of Biomedical Engineering University of Delaware Newark Delaware 19716"},{"name":"Delaware Biotechnology Institute, 15 Innovation Way Newark Delaware 19711"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2004.12.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvoice.2012.03.009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2008.0588"},{"key":"e_1_2_7_5_1","first-page":"474","article-title":"Vocal fold scarring: Current concepts and management","volume":"115","author":"Benninger MS","year":"1996","journal-title":"Otolaryngol Neck Surg"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348949610500303"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3342\/ceo.2010.3.4.177"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e3283407d87"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.08.054"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/biom.23799"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201003963"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2016.02.008"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/7\/2\/024103"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1242\/jeb.37.4.889","article-title":"A rubber\u2010like protein in insect cuticles","volume":"37","author":"Weis\u2010Fogh T.","year":"1960","journal-title":"J Exp Biol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04085"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/mz4002194"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1467-8039(00)00025-6"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-6-41"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.asd.2004.05.006"},{"key":"e_1_2_7_21_1","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1242\/jeb.198.4.1001","article-title":"The role of the timbal in cicada sound production","volume":"198","author":"Young D","year":"1995","journal-title":"J Exp Biol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1242\/jeb.200.11.1681","article-title":"Tymbal mechanics and the control of song frequency in the cicada Cyclochila australasiae","volume":"200","author":"Bennet\u2010Clark HC.","year":"1997","journal-title":"J Exp Biol"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1242\/jeb.201.5.717","article-title":"Asymmetry of tymbal action and structure in a cicada: A possible role in the production of complex songs","volume":"201","author":"Fonseca PJ","year":"1998","journal-title":"J Exp Biol"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09024"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3BM60333D"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6BM00472E"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm2005388"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b00589"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.7b00353"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.01.002"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.5b01014"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b00294"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.5b01255"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.6b00076"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm200373p"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2SM26812D"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2014.00021"},{"key":"e_1_2_7_38_1","first-page":"2008","article-title":"High\u2010frequency viscoelastic shear properties of vocal fold tissues: Implications for vocal fold tissue engineering. Tissue Eng","volume":"18","author":"Teller SS","year":"2012","journal-title":"Part A"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11340-008-9126-4"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201500411"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0892-1997(02)00078-4"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2015.0168"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-015-3668-8"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489402111S1203"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/marc.201400521"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900460"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.06.010"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm061059m"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.05.038"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.6b00500"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2600"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4290"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3462"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2008.00410.x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2007.11.004"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4133"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940811700310"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.01.032"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.07.009"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0802397"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.03.024"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20174"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030965"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2113"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.36418","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T19:35:19Z","timestamp":1694979319000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.36418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,11]]},"references-count":63,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1002\/jbm.a.36418"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.36418","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,11]]},"assertion":[{"value":"2017-06-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}