{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:08:45Z","timestamp":1790759325366,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T00:00:00Z","timestamp":1536710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recessive dystrophic Epidermolysis Bullosa (RDEB) is caused by mutations in collagen\u2010type VII gene critical for the dermoepidermal junction (DEJ) formation. Neither tissues of animal models nor currently available <jats:italic>in vitro<\/jats:italic> models are amenable to the quantitative assessment of mechanical adhesion between dermal and epidermal layers. Here, we created a 3D <jats:italic>in vitro<\/jats:italic> DEJ model using extracellular matrix (ECM) proteins of the DEJ anchored to a poly(ethylene glycol)\u2010based slab (termed ECM composites) and seeded with human keratinocytes and dermal fibroblasts. Keratinocytes and fibroblasts of healthy individuals were well maintained in the ECM composite and showed the expression of collagen type VII over a 2\u2010week period. The ECM composites with healthy keratinocytes and fibroblasts exhibited yield stress associated with the separation of the model DEJ at 0.268 \u00b1 0.057 kPa. When we benchmarked this measure of adhesive strength with that of the model DEJ fabricated with cells of individuals with RDEB, the yield stress was significantly lower (0.153 \u00b1 0.064 kPa) consistent with our current mechanistic understanding of RDEB. In summary, a 3D <jats:italic>in vitro<\/jats:italic> model DEJ was developed for quantification of mechanical adhesion between epidermal\u2010 and dermal\u2010mimicking layers, which can be utilized for assessment of mechanical adhesion of the model DEJ applicable for Epidermolysis Bullosa\u2010associated therapeutics. \u00a9 2018 The Authors. <jats:italic>Journal Of Biomedical Materials Research Part A<\/jats:italic> Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3231\u20133238, 2018.<\/jats:p>","DOI":"10.1002\/jbm.a.36519","type":"journal-article","created":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T20:29:30Z","timestamp":1536784170000},"page":"3231-3238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A 3D <i>in vitro<\/i> model of the dermoepidermal junction amenable to mechanical testing"],"prefix":"10.1002","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5783-4549","authenticated-orcid":false,"given":"Jangwook P.","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Stem Cell Institute, University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Department of Biological Engineering Louisiana State University  Baton Rouge Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Han","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan J.","family":"Riddle","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0957-4380","authenticated-orcid":false,"given":"Jakub","family":"Tolar","sequence":"additional","affiliation":[{"name":"Stem Cell Institute, University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Department of Pediatrics University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Masonic Cancer Center University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda M.","family":"Ogle","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Stem Cell Institute, University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Masonic Cancer Center University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Lillehei Heart Institute University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"},{"name":"Institute for Engineering in Medicine, University of Minnesota\u2010Twin Cities  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcibr1414709"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12277331"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.334"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.75"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.234"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-06-161299"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34292"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.172"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0784"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077673"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.08.017"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5IB00238A"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201200262"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2015.0139"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0479"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm400728e"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.jmbbm.2013.01.024","article-title":"Large amplitude oscillatory shear properties of human skin","volume":"28","author":"Lamers E","year":"2013","journal-title":"J Mech Behav Biomed Mater"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.90"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.178.4063.871"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.03.009"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp5000295"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-009-9325-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6LC00229C"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.31981"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2008.09.006"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.647"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108779200"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113024"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.336073"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24487"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18705"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0ib00112k"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009540"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.523"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.346"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/npjregenmed.2016.14"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt511"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-0358.70712"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/3\/032001"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.388"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-92"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08114.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2012.01482.x"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.36519","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36519","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T11:48:06Z","timestamp":1694778486000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.36519"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,12]]},"references-count":43,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1002\/jbm.a.36519"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.36519","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,12]]},"assertion":[{"value":"2018-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}