{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T06:28:07Z","timestamp":1776752887534,"version":"3.51.2"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004955","name":"Austrian Research Promotion Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004955","name":"\u00d6sterreichische Forschungsf\u00f6rderungsgesellschaft","doi-asserted-by":"publisher","award":["853482"],"award-info":[{"award-number":["853482"]}],"id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microneedles are promising devices for transdermal delivery and diagnostic applications, due to their minimally invasive and painless nature of application. However, so far, applications are limited to small scale research projects. Material selection and production for larger projects remain a challenge. <jats:italic>In vitro<\/jats:italic> testing using human cell culture could bridge the gap between cost effective screening of suitable materials and concerns for safety and ethics. In this study, materials were tested for effects on viability and morphology of human endothelial cells and keratinocytes. In addition, materials were assessed for their potential to influence cellular differentiation and barrier formation. Elution\u2010based testing of inflammatory markers revealed no negative effects in all applied tests, whereas the assessment of differentiation markers on cells in direct contact with the material showed differences and allowed the selection of candidate materials for future medical device applications. This study illustrates that elution\u2010based biocompatibility testing can paint an incomplete picture. Advanced staining techniques and cell types specific for the application of the medical device improve material selection to reduce and replace animal testing at an early stage in the development process. \u00a9 2018 The Authors. <jats:italic>journal Of Biomedical Materials Research Part A<\/jats:italic> Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 505\u2013512, 2019.<\/jats:p>","DOI":"10.1002\/jbm.a.36565","type":"journal-article","created":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T03:46:54Z","timestamp":1542685614000},"page":"505-512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells"],"prefix":"10.1002","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1202-5961","authenticated-orcid":false,"given":"Klaudia","family":"Schossleitner","sequence":"first","affiliation":[{"name":"Skin and Endothelium Research Division, Department of Dermatology Medical University Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conor","family":"O'Mahony","sequence":"additional","affiliation":[{"name":"Tyndall National Institute University College Cork  Cork, T12 R5CP Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Brandst\u00e4tter","sequence":"additional","affiliation":[{"name":"Stratec Consumables GmbH  Anif Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Haslinger","sequence":"additional","affiliation":[{"name":"Functional Surfaces and Nanostructures, Profactor GmbH  Steyr\u2010Gleink Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Demuth","sequence":"additional","affiliation":[{"name":"Skin and Endothelium Research Division, Department of Dermatology Medical University Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Fechtig","sequence":"additional","affiliation":[{"name":"Functional Surfaces and Nanostructures, Profactor GmbH  Steyr\u2010Gleink Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Petzelbauer","sequence":"additional","affiliation":[{"name":"Skin and Endothelium Research Division, Department of Dermatology Medical University Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-chembioeng-060816-101514"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2017.02.011"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/10717541003667798"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2013.05.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1149\/2.0241807jss"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1088\/0960-1317\/26\/8\/084005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1149\/2.0141510jss"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-018-0194-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-010-0177-8"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics7040471"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10404-010-0605-4"},{"key":"e_1_2_8_13_1","volume-title":"Fabrication and analysis of injection molded plastic microneedle arrays","author":"Hamilton JD","year":"2011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00542-006-0204-1"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2010.01.006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36199"},{"key":"e_1_2_8_17_1","unstructured":"Pharmacopeia US.< 87> Biological reactivity tests In Vitro. USP Rockville MD USA.2015;"},{"key":"e_1_2_8_18_1","unstructured":"European Standards.ISO 10993\u20105 Biological Evaluation of Medical Devices \u2010 Part 5: Tests for in vitro Cytotoxicity.2009;"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000178866"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35121"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3281"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2014.10.018"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2868"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.01.004"},{"key":"e_1_2_8_25_1","unstructured":"ZuangV. BarrosoJ. BelzS. BerggrenE. BernasconiC. BoppS. BouhifdM. BoweG. CampiaI. CasatiS. CoeckeS.CR DuraA. GribaldoL. GrignardE. HalderM. HolleyT. Janusch RoiA. KienzlerA. LostiaA. MadiaF. MilcampsA.MS MunnS. PainiA. PistollatoF. PriceA. Prieto\u2010PeraitaP. RicharzA. TriebeJ. van derLindenS. WittwehrC. WorthA.WM.EURL ECVAM Status Report on the Development Validation and Regulatory Acceptance of Alternative Methods and Approaches (2017) [Internet]. EUR 28823. Luxembourg: Publications Office of the European Union;2017."},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201300220"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.2002.2434"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009705200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1310"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.1894"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.36565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T12:15:49Z","timestamp":1694693749000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.36565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,19]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/jbm.a.36565"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.36565","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,19]]},"assertion":[{"value":"2018-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}