{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:37:04Z","timestamp":1790062624303,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,25]],"date-time":"2019-02-25T00:00:00Z","timestamp":1551052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004562","name":"French Ministry of Higher Education and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004562","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Angiogenesis is a critical parameter to consider for the development of tissue\u2010engineered bone substitutes. The challenge is to promote sufficient vascularization in the bone substitute to prevent cell death and to allow its efficient integration. The capacity of nacre extract to restore the osteogenic activity of osteoarthritis osteoblasts has already been demonstrated. However, their angiogenic potential on endothelial progenitor cells (EPCs) was not yet explored. Therefore, the current study aimed at investigating if nacreous molecules affect EPC behavior. The gene and protein expression levels of endothelial cell (EC)\u2010specific markers were determined in EPCs cultivated in presence of a nacre extract (ethanol soluble matrix [ESM] at two concentrations: 100\u2009\u03bcg\/mL and 200\u2009\u03bcg\/mL (respectively abbreviated ESM100 and ESM200)). Cell functionality was explored by proangiogenic factors production and i<jats:italic>n vitro<\/jats:italic> tube formation assay. ESM200 increased the expression of some EC\u2010specific genes. The <jats:italic>in vitro<\/jats:italic> tube formation assay demonstrated that ESM200 stimulated tubulogenesis affecting angiogenic parameters. We demonstrated that a stimulation with 200\u2009\u03bcg\/mL of ESM increased angiogenesis key elements. This <jats:italic>in vitro<\/jats:italic> study strongly highlights the proangiogenic effect of ESM. Due to its osteogenic properties, previously demonstrated, ESM could constitute the key element to develop an ideal prevascularized bone substitute. \u00a9 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.<\/jats:p>","DOI":"10.1002\/jbm.a.36655","type":"journal-article","created":{"date-parts":[[2019,2,9]],"date-time":"2019-02-09T05:04:58Z","timestamp":1549688698000},"page":"1406-1413","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The effect of nacre extract on cord blood\u2010derived endothelial progenitor cells: A natural stimulus to promote angiogenesis?"],"prefix":"10.1002","volume":"107","author":[{"given":"Anne\u2010Sophie","family":"Willemin","sequence":"first","affiliation":[{"name":"Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS\u2010Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010L\u00e8s\u2010Nancy, F\u201054505 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganggang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Velot","sequence":"additional","affiliation":[{"name":"Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS\u2010Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010L\u00e8s\u2010Nancy, F\u201054505 France"},{"name":"Facult\u00e9 de Pharmacie  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy, F\u201054505 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS\u2010Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010L\u00e8s\u2010Nancy, F\u201054505 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Decot","sequence":"additional","affiliation":[{"name":"Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS\u2010Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010L\u00e8s\u2010Nancy, F\u201054505 France"},{"name":"CHRU de Nancy Unit\u00e9 de Th\u00e9rapie Cellulaire et Tissus  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy, F\u201054505 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marthe","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, UJM\u2010Saint Etienne, INSERM, SAINBIOSE U1089  Saint\u2010Etienne, F\u201042000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Gillet","sequence":"additional","affiliation":[{"name":"Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS\u2010Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010L\u00e8s\u2010Nancy, F\u201054505 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Moby","sequence":"additional","affiliation":[{"name":"Ing\u00e9nierie Mol\u00e9culaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS\u2010Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010L\u00e8s\u2010Nancy, F\u201054505 France"},{"name":"CHRU de Nancy\u2010Brabois, Service Odontologie  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy, F\u201054500 France"},{"name":"Facult\u00e9 d'Odontologie Universit\u00e9 de Lorraine  Vand\u0153uvre\u2010l\u00e8s\u2010Nancy, F\u201054505 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(03)02866-6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1365.035"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2012.03.021"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1184-8"},{"key":"e_1_2_7_6_1","first-page":"Doc21","article-title":"Clinical results of autologous bone augmentation harvested from the mandibular ramus prior to implant placement. An analysis of 104 cases","volume":"5","author":"Sakkas A","year":"2016","journal-title":"GMS Interdiscip Plast Reconstr Surg DGPW"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v6.i10.754"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevBiomedEng.v40.i5.10"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2017.00017"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.23306"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2009.07.001"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00106"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2015.29"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-012-9689-z"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v027a01"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-014-0285-y"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4155\/tde.12.50"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000345108"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2741\/s321"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.03.003"},{"key":"e_1_2_7_21_1","first-page":"1","article-title":"The water\u2010soluble matrix fraction from the nacre of Pinctada maxima produces earlier mineralization of MC3T3\u2010E1 mouse pre\u2010osteoblasts","volume":"135","author":"Rousseau M","year":"2003","journal-title":"Comp Biochem Physiol B Biochem Mol Biol"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31553"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2015.2315"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35939"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(99)00141-6"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00213-1"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.12.2232"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0764-4469(97)86933-8"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.098"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2298\/SARH0910518V"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.10.012"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2016.03.016"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.02.012"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.2685"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.036"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2010.00722.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.11.7488"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0267-6605(90)90022-N"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(92)90037-8"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316881"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-1088-4"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.151456"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwu094"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1006\/exmp.2002.2424"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20167144"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3176"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2012.721130"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34629"},{"key":"e_1_2_7_50_1","first-page":"601","article-title":"Repair of critical size bone defects with porous poly(D,L\u2010lactide)\/nacre nanocomposite hollow scaffold","volume":"33","author":"Xiao W\u2010D","year":"2012","journal-title":"Saudi Med J"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143713"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.36655","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36655","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.a.36655","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36655","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T14:23:04Z","timestamp":1694355784000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.36655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,25]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/jbm.a.36655"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.36655","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,25]]},"assertion":[{"value":"2018-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}