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Therefore, we aimed to efficiently accumulate human MSC (hMSC) to damaged brain area to improve the therapeutic effect using poly(ethylene glycol) (PEG)\u2010conjugated phospholipid (PEG\u2010lipid) carrying an oligopeptide as a ligand, specific for E\u2010selectin which is upregulated on activated endothelial cells under hypoxia\u2010like stroke. Here we synthesized E\u2010selectin\u2010binding oligopeptide (ES\u2010bp) conjugated with PEG spacer having different molecular weights from 1 to 40 kDa. We found that ES\u2010bp can be immobilized onto the hMSC surface through PEG\u2010lipid without influence on cell growth and differentiation into adipocytes and osteocytes, respectively. It is also possible to control the immobilization of ES\u2010bp on hMSC surface (&lt;10<jats:sup>8<\/jats:sup> ES\u2010bp per cell). Immobilized ES\u2010bp can be continuously immobilized at the outside of cell membrane when PEG\u2010lipids with PEG 5 and 40 kDa were used. In addition, the modified hMSC can specifically attach onto E\u2010selectin\u2010immobilized surface as a model surface of activated endothelium in human blood, indicating the sufficient number of immobilized ES\u2010bp onto hMSC. Thus, this technique is one of the candidates for hMSC accumulation to cerebral infarction area. \u00a9 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1779\u20131792, 2019.<\/jats:p>","DOI":"10.1002\/jbm.a.36697","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T06:46:29Z","timestamp":1555310789000},"page":"1779-1792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Modification of human MSC surface with oligopeptide\u2010PEG\u2010lipids for selective binding to activated endothelium"],"prefix":"10.1002","volume":"107","author":[{"given":"Makoto","family":"Noiri","sequence":"first","affiliation":[{"name":"Department of Bioengineering School of Engineering, The University of Tokyo  7\u20103\u20101 Hongo, Bunkyo\u2010ku, Tokyo, 113\u20108656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenta","family":"Asawa","sequence":"additional","affiliation":[{"name":"Department of Bioengineering School of Engineering, The University of Tokyo  7\u20103\u20101 Hongo, Bunkyo\u2010ku, 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113\u20108656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Bioengineering School of Engineering, The University of Tokyo  7\u20103\u20101 Hongo, Bunkyo\u2010ku, Tokyo, 113\u20108656 Japan"},{"name":"Department of Material Engineering School of Engineering, The University of Tokyo  7\u20103\u20101 Hongo, Bunkyo\u2010ku, Tokyo, 113\u20108656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina N","family":"Ekdahl","sequence":"additional","affiliation":[{"name":"Linnaeus Center of Biomaterials Chemistry Linnaeus University  SE\u2010391 82, Kalmar Sweden"},{"name":"Department of Immunology, Genetics, and Pathology (IGP) Uppsala University  Dag Hammarskj\u00f6lds v\u00e4g 20, SE\u2010751 85, Uppsala 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