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Sclerostin single\u2010chain antibody fragments (SCL\u2010scFv) have been proven to promote bone formation by binding to scleroprotein, a natural antagonist of the Wnt pathway, but it is difficult to rule alone due to the weak permeability and immunogenicity. Herein, we prepared poly(lactic\u2010co\u2010glycolic acid) microspheres as a sustained\u2010release vehicle to prolong the activity of SCL\u2010scFv. The morphology of microspheres were uniform and nearly sphere, loading efficiency and encapsulation efficiency of SCL\u2010scFv microspheres were 6.28\u2009\u00b1 1.04% and 48.37\u2009\u00b1 8.11%, respectively. Approximately 90% of the SCL\u2010scFvs were released from the microspheres over 28\u2009days with initial burst releasing (38%) in the first 4\u2009days. Sustained\u2010release of active SCL\u2010scFv from microspheres promoted bone marrow mesenchymal stem cells osteogenic differentiation in vitro and enhanced fracture healing in ovariectomized rats by improving bone mass and bone formation in the fracture region. All these findings demonstrate that the microspheres are able to simultaneously achieve localized long\u2010term SCL\u2010scFv controlled release and effectively promote bone formation, which provides a promising approach for osteoporotic fracture.<\/jats:p>","DOI":"10.1002\/jbm.a.36704","type":"journal-article","created":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T08:56:05Z","timestamp":1556009765000},"page":"1832-1840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Sustained\u2010release of sclerostin single\u2010chain antibody fragments using poly(lactic\u2010co\u2010glycolic acid) microspheres for osteoporotic fracture repair"],"prefix":"10.1002","volume":"107","author":[{"given":"Ming","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Chinese PLA General Hospital  Beijing 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