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A novel platelet concentrate termed i\u2010PRF (injectable platelet\u2010rich fibrin) has recently been developed without any additives by low centrifugation speeds. To date, scientists have investigated the capability of releasing growth factors to improve regeneration but have ignored whether i\u2010PRF can inhibit the inflammatory effect around the wound. The present study investigated the anti\u2010inflammation effects of i\u2010PRF on immune response\u2010related cells, especially macrophages and dendric cells. We found that i\u2010PRF reduced pro\u2010inflammatory M1 phenotype of macrophages and activated dendritic cells around muscle defect that was injected with bacterial suspension. Moreover, in vitro experiments showed similar results. i\u2010PRF deleted inflammatory response caused by lipopolysaccharide to some extent. We determined that TLR4, an activator of inflammatory stimulation and p\u2010p65, a key factor belongs to classical inflammatory related NF\u2010\u03baB signal pathway, can be inhibited by use of i\u2010PRF. Results indicate the potential anti\u2010inflammatory role of i\u2010PRF during regeneration and restoration.<\/jats:p>","DOI":"10.1002\/jbm.a.36792","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T14:58:15Z","timestamp":1566831495000},"page":"61-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":86,"title":["Anti\u2010inflammation effects of injectable platelet\u2010rich fibrin via macrophages and dendritic cells"],"prefix":"10.1002","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1998-8851","authenticated-orcid":false,"given":"Jinglun","family":"Zhang","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) and Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology, Wuhan University  Wuhan China"},{"name":"Department of Oral Implantology School and Hospital 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Hospital of Stomatology, Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zifan","family":"Zhao","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) and Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology, Wuhan University  Wuhan China"},{"name":"Department of Oral Implantology School and Hospital of Stomatology, Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyang","family":"Wang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) and Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology, Wuhan University  Wuhan China"},{"name":"Department of Oral Implantology School and Hospital of Stomatology, Wuhan University  Wuhan 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