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The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X\u2010ray diffraction, confocal microscopy, adsorption\/desorption of nitrogen, rheometry, and 3\u20104,[5\u2010dimethylthiazol\u20102\u2010yl]\u20102,5\u2010diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively. The samples showed a decomposition onset temperature below 253.3\u2009\u00b1\u20094.8\u00b0C, a semicrystalline nature, and a highly porous structure. Hydrogels reached the maximum water uptake in phosphate\u2010buffered saline after 80\u2009min, showing values from nine to  twelve times their dry mass. Also, hydrogels exhibiting a solid\u2010like behavior ranging from 2,567 \u00b1\u2009467 to 48,705 \u00b1\u20092,453 Pa at 0.1 rad\/s (low frequency). The sterilized hydrogels showed low cytotoxicity (cell viability\u2009&gt;\u200970%) to the HT29\u2010MTX\u2010E12 cell line. Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.<\/jats:p>","DOI":"10.1002\/jbm.a.36794","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T08:33:18Z","timestamp":1568104398000},"page":"81-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Sterilized chitosan\u2010based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity"],"prefix":"10.1002","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3383-985X","authenticated-orcid":false,"given":"Rogelio","family":"Rodr\u00edguez\u2010Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Biotecnolog\u00eda M\u00e9dica y Farmac\u00e9utica CIATEJ  Guadalajara Jalisco 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