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A novel peptide PTHrP\u20101 was derived from PTH and used at different concentrations (0, 0.1, 0.3, and 0.5 mg\/ml) to induce ectopic osteogenesis. Radiographic examinations (X\u2010ray, CT, and 3D reconstruction), pathological observations (H&amp;E, Masson, Von Kossa, ALP and TRAP staining), immunohistochemical staining (Col\u2010I, OCN and Runx\u20102 in tissues), western blotting was used to determine the qualitative and quantitative analysis of related markers proteins. Results confirmed that the appropriate concentration of PTHrP\u20101 can effectively enhance the osteogenic activity, thereby improving the positive results and protein expression of osteogenic markers (COL\u2010I, OCN, and Runx\u20102) in the quadriceps muscle pocket of SD rats. Through qualitative and quantitative analysis, high concentration of PTHrP\u20101 promotes osteogenic activity and active bone resorption. This study confirmed that PTHrP\u20101 is a novel small molecule bioactive peptide, and the rat tail collagen scaffold is a good carrier of PTHrP\u20101 with excellent biocompatibility. The PTHrP\u20101\/Col\u2010I composite scaffold material is an effective substitute for bone tissue engineering and can effectively induce and promote bone formation in the quadriceps muscle pocket of rats. In addition, the promoting ability for osteogenic differentiation of 0.3 mg\/ml PTHrP\u20101\/Col\u2010I composite scaffold material group was significantly better than that of 0, 0.1 and 0.5 mg\/ml PTHrP\u20101\/Col\u2010I composite scaffold material groups. Hence, the optimal concentration of PTHrP\u20101 to promote ectopic osteogenesis is 0.3 mg\/ml.<\/jats:p>","DOI":"10.1002\/jbm.a.36802","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T23:39:41Z","timestamp":1570145981000},"page":"166-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Ectopic osteogenesis by type I collagen loaded with a novel synthesized PTH\u2010related peptide\u20101 in vivo"],"prefix":"10.1002","volume":"108","author":[{"given":"Yi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Zhongnan Hospital of Wuhan University  Wuhan Hubei People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Zhongnan Hospital of Wuhan University  Wuhan Hubei People's Republic of 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