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Recent interest in using pectin polysaccharides as mesothelial sealants suggests that water content, rather than nonphysiologic changes in temperature, may be a practical approach to optimize the physical properties of the pectin biopolymers. Here, we used humidified environments to manipulate the water content of dispersed solution of pectins with a high degree of methyl esterification (high\u2010methoxyl pectin; HMP). The gel phase transition was identified by a nonlinear increase in compression resistance at a water content of 50% (w\/w). The gel phase was associated with a punched\u2010out fracture pattern and scanning electron microscopy (SEM) images that revealed a cribiform (Swiss cheese\u2010like) pectin microstructure. The glass phase transition was identified by a marked increase in resilience and stiffness. The glass phase was associated with a star\u2010burst fracture pattern and SEM images that demonstrated a homogeneous pectin microstructure. In contrast, the burst strength of the pectin films was largely independent of water content over a range from 5 to 30% (w\/w). These observations indicate the potential to use water content in the selective regulation of the physical properties of HMP biopolymers.<\/jats:p>","DOI":"10.1002\/jbm.a.36811","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:28:14Z","timestamp":1570580894000},"page":"246-253","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Pectin biopolymer mechanics and microstructure associated with polysaccharide phase transitions"],"prefix":"10.1002","volume":"108","author":[{"given":"Aidan","family":"Pierce","sequence":"first","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willi L.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School  Boston Massachusetts"},{"name":"Department of Diagnostic and Interventional Radiology Translational Lung Research Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik V.","family":"Scheller","sequence":"additional","affiliation":[{"name":"The Joint BioEnergy Institute and Environmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory  Berkeley California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debra","family":"Mohnen","sequence":"additional","affiliation":[{"name":"Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Tsuda","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Physiological Sciences Harvard School of Public Health  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy University Medical Center of the Johannes Gutenberg\u2010University  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Mentzer","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology Brigham &amp; Women's Hospital, Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.03.051"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-3861(97)00119-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042811-105534"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2012.00092"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-017-0866-1"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1325-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.93.158105"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp207261r"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/la103487a"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.09.062"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2010.10.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pi.4990250302"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2017.05.029"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2008.05.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfoodeng.2007.03.012"},{"key":"e_1_2_7_17_1","volume-title":"Colloid science","author":"Hermans P. 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