{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:34:50Z","timestamp":1761514490743,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hypothyroidism is an autoimmune disease associated with underactive thyroid gland. In this study, a dual effect polymeric system was designed to release Cepharanthine (CEP) to block T cell activation and Selenium (Se) to decrease the anti\u2010thyroid peroxidase (TPOAb) concentration in order to treat hypothyroidism. For this purpose, poly(ethylene\u2010vinyl acetate) (PEVA) and polyethylene glycol (PEG) nanoparticles (NPs) including CEP were synthesized by emulsion solvent evaporation method and they were loaded to polyurethane (PU)\/PEG\u2010PUSe\u2010PEG block copolymer blends which were fabricated by particulate leaching technique as porous sponges. Fourier\u2010Transform Infrared (FTIR), Raman, and Nuclear Magnetic Resonance (NMR) analysis showed successful synthesis of PEG\u2010PUSe\u2010PEG block copolymer. A long\u2010term zero\u2010order release profile was obtained for CEP. Se release rate from matrices showed an oxidative stress\u2010mediated release which can be used to adjust Se amount. According to MTS results conducted by NIH 3T3 fibroblasts, both NPs and matrices have no adverse effect on cell viability. Fluorescence microscopy and SEM images confirm the MTS results. The dual release system has potential to be effectively used in long\u2010term treatment of hypothyroidism by addressing both auto\u2010immune response and hormone regulation.<\/jats:p>","DOI":"10.1002\/jbm.a.36855","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T21:51:20Z","timestamp":1575323480000},"page":"760-759","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A multifunctional long\u2010term release system for treatment of hypothyroidism"],"prefix":"10.1002","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-3618","authenticated-orcid":false,"given":"Ceren","family":"Kutlu Kaya","sequence":"first","affiliation":[{"name":"NanoScience &amp; Nanoengineering Program Istanbul Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selin","family":"G\u00fcmr\u00fck\u00e7\u00fc","sequence":"additional","affiliation":[{"name":"Chemistry Department Istanbul Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulkadir Sezai","family":"Sara\u00e7","sequence":"additional","affiliation":[{"name":"NanoScience &amp; Nanoengineering Program Istanbul Technical University  Istanbul Turkey"},{"name":"Polymer Science and Technology Istanbul Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5093-6013","authenticated-orcid":false,"given":"Fatma Ne\u015fe","family":"K\u00f6k","sequence":"additional","affiliation":[{"name":"NanoScience &amp; Nanoengineering Program Istanbul Technical University  Istanbul Turkey"},{"name":"Molecular Biology &amp; Genetics Department Istanbul Technical University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,11]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"26","article-title":"Cracking the code for thyroid hormone signaling","volume":"124","author":"Bianco A. C.","year":"2013","journal-title":"Transactions of the American Clinical and Climatological Association"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2013-1083"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI60047"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00119"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1497.1997.07109.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30703-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0321"},{"key":"e_1_2_7_9_1","unstructured":"Dojindo.com. Cell Counting Kit\u20108 General Information Cell Proliferation Assay and Cytotoxicity Assay Technical Manual [Updated2016February 17]. Available from:https:\/\/www.dojindo.com\/TechnicalManual\/Manual_CK04.pdf."},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-99079-8"},{"issue":"3","key":"e_1_2_7_11_1","first-page":"37","article-title":"Treatment of hypothyroidism","volume":"7","author":"Ersoy C. O.","year":"2014","journal-title":"Turkiye Klinikleri Journal of Endocrinology Special Topics"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60954-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520190309"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.4.8421"},{"volume-title":"Underactive thyroid: Overview","year":"2006","author":"InformedHealth.org","key":"e_1_2_7_15_1"},{"volume-title":"Biological evaluation of medical devices \u2010 Part 1: Evaluation and testing within a risk management process, Guidance for Industry and Food and Drug Administration Staff","year":"2016","author":"ISO 10993\u20101","key":"e_1_2_7_16_1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/02652048.2014.913727"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmmm.2003.12.416"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.694687"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0py00144a"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2007.0169"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000065768.72937.62"},{"key":"e_1_2_7_23_1","first-page":"51","article-title":"Myo\u2010inositol plus selenium supplementation restores euthyroid state in Hashimoto's patients with subclinical hypothyroidism","volume":"21","author":"Nordio M.","year":"2017","journal-title":"European Review for Medical and Pharmacological Sciences"},{"issue":"2","key":"e_1_2_7_24_1","first-page":"252","article-title":"Restrictions in the response to autologous thyroglobulin in the human","volume":"41","author":"Nye L.","year":"1980","journal-title":"Clinical and Experimental Immunology"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2010.09.017"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(11)70500-X"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.15226\/2374-6890\/3\/1\/00143"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/pj.2014.10"},{"key":"e_1_2_7_29_1","unstructured":"Tetteh G. A. J. Polyurethane\u2010based scaffolds for bone tissue engineering. PhD thesis. University of Sheffield United Kingdom;2016 247p."},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2009.02.007"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06661"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3109\/10837450.2013.819011"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.39694"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5457-9"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.05.003"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-017-0693-z"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.a.36855","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.36855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T10:46:53Z","timestamp":1693910813000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.36855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,11]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1002\/jbm.a.36855"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.36855","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"type":"print","value":"1549-3296"},{"type":"electronic","value":"1552-4965"}],"subject":[],"published":{"date-parts":[[2019,12,11]]},"assertion":[{"value":"2019-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}