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SDF\u20101\u03b1\u2010aligned SF microfiber\/3D\u2010BAMG, aligned SF microfiber\/3D\u2010BAMG, and nonaligned SF microfiber\/3D\u2010BAMG scaffolds were prepared using electrostatic spinning and wet processing. Adipose\u2010derived stem cell (ADSC) and bone marrow stromal cell (BMSC) migration was assessed in the SDF\u20101\u03b1\u2010loaded scaffolds. Sustained SDF\u20101\u03b1 release in vitro and vivo was analyzed using enzyme\u2010linked immunosorbent assay (ELISA) and western blotting, respectively. The scaffolds were used to repair a 1.5\u2009\u00d7\u20091 cm<jats:sup>2<\/jats:sup> ventral urethral defect in male rabbits in vivo. General observation and retrograde urinary tract contrast assessment were used to examine urethral lumen patency and continuity at 1 and 3 months post\u2010surgery. Postoperative rehabilitation was evaluated using histological detection. The composite scaffolds sustained SDF\u20101\u03b1 release for over 16\u2009days in vitro. SDF\u20101\u03b1\u2010aligned SF nanofiber promoted regeneration of urethral mucosa, submucosal smooth muscles, and microvasculature, increased cellular proliferation, and reduced collagen deposition. SDF\u20101\u03b1 expression was increased in reconstructed urethra at 3 months post\u2010surgery in SDF\u20101\u03b1\u2010aligned SF group. SDF\u20101\u03b1\u2010aligned SF microfiber\/3D\u2010BAMG scaffolds may be used to repair and reconstruct long urethral defects because they accelerate urethral regeneration.<\/jats:p>","DOI":"10.1002\/jbm.a.36943","type":"journal-article","created":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T21:05:06Z","timestamp":1586552706000},"page":"1760-1773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sustained release of stromal cell\u2013derived factor\u20101 alpha from silk fibroin microfiber promotes urethral reconstruction in rabbits"],"prefix":"10.1002","volume":"108","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai China"},{"name":"Shanghai Eastern Institute of Urologic Reconstruction  Shanghai China"},{"name":"Department of Urology, Weifang People's Hospital Weifang Medical University  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering Donghua University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai China"},{"name":"Shanghai Eastern Institute of Urologic Reconstruction  Shanghai China"},{"name":"Department of Urology, Weifang People's Hospital Weifang Medical University  Weifang Shandong China"},{"name":"State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering Donghua University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubo","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Murphy","sequence":"additional","affiliation":[{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Medical Center Boulevard  Winston\u2010Salem North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Ali","sequence":"additional","affiliation":[{"name":"Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine Medical Center Boulevard  Winston\u2010Salem North Carolina"},{"name":"Department of Chemistry, Faculty of Science Zagazig University  Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaopeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering Donghua University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lujie","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201704636"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2018.04.006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2018.02.3102"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2016.04.025"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(200107)56:1<101::AID-JBM1074>3.0.CO;2-6"},{"key":"e_1_2_8_7_1","first-page":"932","volume-title":"Chemokine Receptor CXCR4. 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